麻竹AP2/ERF类转录因子BCR1调控低温胁迫响应的分子机理研究

低温是影响竹子生长发育与地域分布的主要因子之一，也是限制“南竹北移”工程的主要瓶颈。由于竹类开花的特殊性，很难通过传统的育种方法获得耐冷性提高的新种质资源。如何挖掘重要的功能基因并阐述其作用机理，进而利用基因工程提高竹子的耐寒能力一直是人们关注的问题。本课题组前期通过转录组学分析的方法从麻竹中分离到一个对冷胁迫早期响应的AP2/ERF类转录因子BCR1（Bamboo Cold Response 1），BCR1的表达受冷胁迫的快速诱导，且过量表达BCR1的转基因竹提高了耐冷性。在此基础上本项目拟通过本课题组研发的一系列用于研究竹类功能基因的技术体系，并结合蛋白组学、转录组学、植物生理生化等相关的实验手段系统的研究BRC1参与冷胁迫响应的分子机制。同时，通过基因工程的手段创制具有潜在应用价值的耐寒竹。本项目将增进我们对竹子冷胁迫信号通路的认识，并为将来培育抗逆竹提供基础理论依据和材料储备。

Low temperature is one of the main factors that restrict bamboo growth, development and geographical distribution, also the bottleneck for "South Bamboo Transfer to North” program in China. Classic genetic breeding is not applicable for bamboo due to its long and irregular flowering habit. How to uncover the bamboo cold response pathway, dig out important genes and study their functional mechanisms, and use for genetic breeding is of great theoretical and practical significance. Previously, our group isolated an AP2/ERF transcription factor named BCR1 （Bamboo Cold Response 1）through analyzing RNA-Seq data, which is an early cold-responsive gene in Ma bamboo. Our prelimilary data showed that BCR1 is induced by cold treatment, and overexpression of BCR1 in bamboo leads to the cold tolerant phenotype. This project will systermatically investigate the molecular mechnism of BCR1 in bamboo cold response through a number of platforms we established for bamboo gene function investigation, as well as methods from proteomics, transcriptomics, plant physiology and bio-chemistry. We will try to establish cold-tolerant bamboo that could be potentially used in forestry in the future. Our study will add our knowledge on bamboo cold response and provide new theoretical basis for genetic breeding of bamboo in the future.