蛋白质分离纯化技术是蛋白质产品制备的关键技术之一，也是当前蛋白质应用的一个瓶颈。反胶束液液萃取蛋白质技术因具易于放大、可实现连续操作、反胶束相可循环利用等特点而倍受关注。目前用于蛋白质萃取的表面活性剂多限于传统离子型表面活性剂。最近我们以Gemini阳离子表面活性剂二溴化N,N'-二(二甲基十二烷基)-1,8-己二铵反胶束萃取牛血清白蛋白，发现它表现出诸多一直令人期待的优势：萃取条件温和、体系不易乳化、蛋白质装载量大。本项目拟用Gemini阳离子表面活性剂反胶束从复杂基体中提取多种蛋白质，深入系统地研究该类反胶束对蛋白质的萃取效能、萃取过程中反胶束微观结构变化和两相界面行为变化、萃取过程对蛋白质结构和活性的影响，探究萃取机理，根据目标蛋白质性质设计、优化Gemini表面活性剂结构和反胶束组成，实现复杂基体中不同种类蛋白质的高效提取，为蛋白质分离纯化提供切实可行的解决思路与方案。

Separation and purification of protein, a key to the preparation of protein, is currently limiting its application. Liquid-liquid reverse micellar extraction can be easily scaled up and operated in continuous mode with reverse micelle used repeatedly, and hence receives much concern. So far conventional ionic surfactants di-2-ethylhexyl sodium sulfosuccinate and cetyl trimethyl ammonium bromide have been almost exclusively used in reverse micellar extraction. Recently, we extracted bovine serum albumin with gemini cationic surfactant octamethylene-1,8-bis(dimethyl dodecyl ammonium bromide) (12-8-12) reverse micelle and found 12-8-12 displayed distinct advantages over monomeric surfactant in the liquid-liquid reverse micellar extraction process: 1) the extraction condition with 12-8-12 reverse micelle was much milder, 2) the extration system with 12-8-12 reverse micelle was not emulsified, and 3) the loading capacity of 12-8-12 reverse micelle was much higher. In this project we will extract different proteins with gemini cationic surfactant reverse micelles from natural products, and study 1) the extraction efficiencies of different proteins, 2) the changes of reverse micellar structure and oil/water interfacial property during extraction, and 3) the effect of extraction process on the structure and activity of protein. We will further propose the extraction mechanism and optimize the gemini surfactant structure and reverse micelle composition according to the property of the target protein. This research should be meaningful for the development of the technique for protein separation and purification.