随着癌症发病率和死亡率的不断提高,癌症的早期诊断及有效治疗已经成为当今研究的难点和热点问题。目前直接的肿瘤标志物的检测存在着敏感性和特异性差的问题，本课题围绕这一问题展开，借助多功能的PAMAM纳米材料，通过修饰叶酸提高其肿瘤靶向，通过与Cu2+形成配合物放大检测信号（PAMAM:Cu2+为1：20），借助上海同步辐射光源的硬X射线微聚焦线站通过检测Cu荧光强度及分布情况来研究该配合物对肿瘤细胞的识别及成像。研究结果不仅建立一种用硬X射线荧光进行肿瘤细胞及亚细胞成像的方法，而且为后续基于PAMAM的抗肿瘤药物的靶向运输及64Cu等放射性同位素的肿瘤PET成像及治疗提供借鉴。在用同步辐射光源进行肿瘤细胞成像方面、抗肿瘤药物的靶向输送方面及纳米树状分子的应用领域都具有重大的意义和研究价值。

With increased cancer morbidity and mortality，early diagnosis and efficient treatment of cancer has become crucial objective. Detection of tumor markers have poor sensitivity and specificity，the multi-functional nano-materials can improve the detection sensitivity and specificity, combined with high resolution and highly sensitive detection devices, the disease development in the sub-micro cell structure imaging and the microscopic mechanism can be revealed. This project intends to make use of PAMAM Dendrimers modified with tumor target molecules group and complexed with Cu2+ to recognize cancer cell and image by synchrotron X-ray fluorescence microprobe. The research will establish a method of using hard X-ray fluorescence imaging of tumor cells, and provide a reference for the PET imaging and treatment of tumors based on PAMAM and 64Cu radioisotope. It is helpful in tumor cell imaging with synchrotron radiation X-ray, targeted transportation of anticancer drugs and applications of dendrimers