CSA是临床常见病、慢性病，严重影响国民身心健康。CSA发病原因之一是血管内皮细胞形态结构的损伤。自噬对维持血管内环境稳态具有十分重要的作用，自噬的调节与多个通路、多种因子相关。项目组在前期研究基础上提出“自噬在CSA病理机制中具有重要作用，特定基线的适度自噬可维持血管内环境稳态，活血定眩胶囊通过Bcl-2与PI3K/Akt/mTOR信号通路间的‘串话’调控血管内皮细胞自噬水平而达到治疗CSA之目的”的工作假说。为此，拟开展：①离体培养bEnd.3细胞，采用乏氧刺激、siRNA转染等方法，运用RT-PCR、Western Blot及免疫共沉淀技术，探讨Bcl-2与PI3K/Akt/mTOR信号通路间“串话”对自噬的调控机制；②在体建立CSA大鼠模型，采用透射电镜及生物学检测探讨活血定眩胶囊对血管内皮细胞自噬功能的调节机制。最终在细胞和基因水平上为中医药防治CSA提供研究思路，指导临床用药。

CSA is a common clinical disease and chronic disease, which seriously affects the physical and mental health of the citizen. One of the causes of CSA is the damage of morphology and structure of vascular endothelial cells. Autophagy plays an important role in maintaining the homeostasis of blood vessels, The regulation of autophagy is associated with multiple pathways and multiple factors. Based on the previous study, we suggest that Autophagy plays an important role in the pathogenesis of CSA, and proper autophagy at a specific baseline can maintain intravascular environmental homeostasis, Through the "crosstalk" between Bcl-2 and PI3K / Akt / mTOR signal pathway, Huoxue Dingxuan Capsule regulates the autophagy of vascular endothelial cells to reach the working hypothesis for the treatment of CSA. Therefore, we intend to carry out: ①In vitro experiments: by culturing bEnd.3 cells, Using hypoxia stimulation, siRNA interference and other methods, Using RT-PCR, Western Blot and immunoprecipitation and other techniques, To explore the regulatory mechanism of Bcl-2 and PI3K/Akt/mTOR signaling pathway between the "crosstalk" on autophagy; ②In vivo experiment: Establishing the CSA rat model, Using transmission electron microscopy, immunohistochemistry and a variety of biological detection, To explore the regulatory mechanism of Huoxue Dingxuan Capsule on the autophagy function of vascular endothelial cells. Ultimately, it provides the research ideas for the prevention and treatment of CSA by Traditional Chinese Medicine at the cell and gene level, and to guide clinical medicine.