植物内生菌是近年发现的一种重要的植物体内环境生态因子，是促进宿主活性成分积累的重要因素之一。宁夏枸杞（Lycium barbarum）是宁夏特色优势产业。本项目拟基于宏基因组DNA，在特异性PCR扩增内生细菌16S rDNA序列和内生真菌ITS序列基础上，运用454焦磷酸高通量测序技术，揭示基于OTU的宁夏枸杞内生菌群落特征；同时，通过典型相关分析和实时荧光定量PCR明确宁夏枸杞活性成分含量相关内生菌群；最后通过回接实验分析内生菌对宿主生长发育、抗逆性及活性成分代谢相关基因表达的影响，阐明内生菌群调控宁夏枸杞有效成分积累的机制，为内生菌应用于宁夏枸杞育种栽培提供支持。

Endophyte is an important environmental factor in plants found in recent years, which can contribute to promoting host active ingredients accumulation. Lycium barbarum is special advantage industry in Ningxia. In this study，the specific PCR and the latest 454-Pyrophosphate sequencing will be used to reveal endophytic community characteristics in Lycium barbarum based on 16S rDNA and ITS. While, the endophyte related content of host active ingredients will be determined with canonical correlation analysis and Real-time quantitative PCR. Finally, the inoculation experiment will be used to analysis effects of endophyte on host growth and development,stress resistance and the expression of gene related active ingredient metabolism，then unvover the mechanism for endophyte regulation active ingredients accumulation in Lycium barbarum.The results of this study can provide a support for application endophyte in Lycium barbarum breeding and cultivation.