rGO通过S1P-S1PR1调节巨噬细胞极化影响材料骨重塑的作用机制研究

批准号:
82002304
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
周凯
依托单位:
学科分类:
骨、关节、软组织损伤与修复
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
周凯
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中文摘要
大段骨缺损会导致患者肢体残疾和劳动能力丧失,临床上常通过植入人工材料进行治疗。目前针对植入材料介导骨重塑机制的认识并不全面,导致许多材料的实际应用效果欠佳。申请人前期研究发现还原性氧化石墨烯(reduced graphene oxide,rGO)具有促进快速骨重塑和调节巨噬细胞极化的作用,但相关机制尚未阐明。结合文献报道和前期结果提示,rGO募集局部环境中的1-磷酸鞘氨醇(sphingosine-1-phosphate,S1P)可能是调控巨噬细胞向M2表型极化并影响材料快速骨重塑的重要机制。本项目以材料骨重塑过程中成骨-免疫-破骨三方相互作用为切入点,通过体内外实验,阐明rGO通过S1P-S1PR1调节巨噬细胞向M2表型极化并影响RANK/RANKL/OPG信号轴对成骨-破骨活动达到动态调控的机理。本项目的实施,将为合理利用rGO的免疫调节特性以研发和改进骨修复材料提供新思路和理论支撑。
英文摘要
Large segment bone defect can lead to limb disability and labor incapacity. In clinical practice, it is often treated by implanting artificial bone repair materials. The current understanding of the mechanism of implant material-mediated bone remodeling is not comprehensive, resulting in poor practical application of many materials. The applicant's previous research found that reduced graphene oxide (rGO) has the effect of promoting rapid bone remodeling and regulating macrophage polarization, but the relevant mechanism has not been elucidated. Combined with literature reports and previous results, we speculate that the recruitment of S1P in the local environment by rGO is a key mechanism for regulating the macrophage polarization to the M2 phenotype and affecting the rapid bone remodeling of the material. In this project, the interaction of osteogenesis, immunity, and osteoclast in the process of bone remodeling was taken as the starting point. In combination with in vivo and in vitro experiments, the mechanism of rGO regulating the polarization of macrophages to M2 phenotype and affecting the dynamic regulation of the RANK/RANKL/OPG signal axis on osteogenic-osteogenesis activity was elucidated by interfering with S1P-S1PR1 signal pathway. The implementation of this project will provide new ideas and theoretical support for the rational use of rGO's immunomodulatory properties to develop and improve bone repair materials.
期刊论文列表
专著列表
科研奖励列表
会议论文列表
专利列表
DOI:10.1038/s41374-021-00595-5
发表时间:2021-04-13
期刊:LABORATORY INVESTIGATION
影响因子:5
作者:Zhou, Kai;He, Sirong;Zhou, Zongke
通讯作者:Zhou, Zongke
DOI:10.3389/fbioe.2023.1185520
发表时间:2023
期刊:Frontiers in bioengineering and biotechnology
影响因子:5.7
作者:
通讯作者:
DOI:10.1016/j.biomaterials.2023.122036
发表时间:2023-02
期刊:Biomaterials
影响因子:14
作者:K. Zhou;Chengli Yang;K. Shi;Yue Liu;D. Hu;Xinlong He;Yun Yang;Bingyang Chu;Jinrong Peng;Zongke Zhou;Z. Qian
通讯作者:K. Zhou;Chengli Yang;K. Shi;Yue Liu;D. Hu;Xinlong He;Yun Yang;Bingyang Chu;Jinrong Peng;Zongke Zhou;Z. Qian
靶向注入功能化修复液对电缆的水树修复及长期作用
- 批准号:--
- 项目类别:面上项目
- 资助金额:54万元
- 批准年份:2022
- 负责人:周凯
- 依托单位:
多应力下电缆附件界面的绝缘老化及修复填充物的作用机理研究
- 批准号:51877142
- 项目类别:面上项目
- 资助金额:62.0万元
- 批准年份:2018
- 负责人:周凯
- 依托单位:
纳米复合填充物对水树的修复及其在多因子老化下的作用机理研究
- 批准号:51477106
- 项目类别:面上项目
- 资助金额:80.0万元
- 批准年份:2014
- 负责人:周凯
- 依托单位:
国内基金
海外基金
