种子休眠指具有正常活力的种子在不适宜环境中不能萌发的现象。种子休眠调控是一项复杂的生理过程，牵涉许多基因表达、植物激素和环境等因素。而种子的早期发育是一个多维度和多模态的空间动态发育过程。以往对于种子休眠与发育的研究多集中于打破休眠方法和激素调控机制等，而对于植物种子内部组织结构动态变化和细胞排布规律在种子休眠与发育调控机制的研究尚未报道。. 前期工作中，我们开发建立了种子三维成像方法，并获取了部分种子不同维度结构与细胞排布。在前期工作基础上，项目将采用Micro CT、LSFM和AutoCUTS-SEM技术对处于休眠、打破休眠及早期发育的种子（拟南芥、加拿大杨和油松）进行宏观、介观和微观尺度的三维重构和分析。在细胞拓扑分析的基础上，结合分子生物学、生物化学与转录组学手段对种子细胞生物网络特征与发育机理进行探索，以期揭示种子组织动态变化调控其休眠与发育的分子细胞学机制。

Seed dormancy refers to the phenomenon that seed with normal vigor cannot germinate in unsuitable environments. Regulation of seed dormancy is a complicated physiological process involving diversified factors such as gene expression, plant hormones, and the external environment. The postembryonic development is a spatial dynamic development process with multi-dimensional and multi-modal. Previous studies on seed dormancy and development have focused on breaking dormancy methods and hormone regulation mechanisms. However, research on the dynamic changes of plant seed internal tissue structure and cell arrangement rules in seed dormancy and development regulation mechanisms has not been reported.. In previous work, we developed and established three-dimensional (3D) imaging methods for seed embryos, and obtained partial information about the internal structure and cell arrangement with multi-dimensional. Based on the previous work, this project will use Micro CT, LSFM and AutoCUTS-SEM 3D imaging technique to carry out macroscopic, mesoscopic and microcosmic 3D reconstruction of seed embryos (Arabidopsis thaliana, Populus × canadensis Moench and Pinus tabulaeformis) at rest, breaking dormancy and early development. Based on the analysis of the cellular network topology, we will investigate the cellular network and development-related mechanisms of plant embryo combined with molecular biology, biochemistry and transcriptomics. These results would reveal the molecular cell biology regulation mechanism of internal organs dynamic changes involved in seed dormancy and development.