组织工程为骨骼肌-肌腱结合处（Muscle-Tendon Junction，MTJ）的损伤修复提供一个新手段。然而，如何制备充分模拟MTJ各向异性移行结构的仿生支架并结合力学拉伸刺激用于组织工程化MTJ的构建仍是研究的热点和难点。申请人前期已利用静电纺丝纤维及水凝胶的仿生支架在构建组织工程化三维骨骼肌、心肌和神经组织相关应用中均展现出良好的潜力。本项目拟采用干湿法静电纺丝技术所制得的取向纳米纤维束与双网络水凝胶相结合，制备模拟MTJ各向异性移行结构的仿生支架，实现基于骨骼肌细胞和肌腱细胞三维共培养的组织工程化MTJ的构建。阐明相关参数对于骨骼肌细胞和肌腱细胞三维生长的促进作用。并进一步对仿生支架施加力学拉伸刺激，探究不同拉伸参数对细胞的成熟和功能化以及对组织工程化MTJ构建的影响。为新型仿生支架用于组织工程化MTJ的构建和MTJ损伤修复应用提供新思路和理论基础。

Tissue engineering strategy has been regarded as a new approach for Muscle-Tendon Junction (MTJ) repair. However, it remains a challenge to develop biomimetic scaffolds that mimicking the anisotropic and transitional structure of MTJ and combined with mechanical stretch for tissue engineered MTJ applications. Our previous studies have demonstrated that the biomimetic composite scaffolds based on electrospun nanofibers and hydrogels showed great potential in skeletal muscle, cardiac muscle and periphery nerve tissue engineering applications. This project aims to fabricate a biomimetic scaffold with the anisotropic and transitional structure that mimicking the complex 3D structure of native MTJ, which based on the aligned nanofiber yarns and double network hydrogel. The tissue engineered MTJ was further developed to provide a 3D co-culture system for muscle cells and tenocytes, the suitable parameters will be identified. Furthermore, the mechanical stretch stimulus is applied to the cell-laden biomimetic scaffolds, and the effects of stretch parameters on tissue engineered MTJ will be investigated. This study will provide new methods and ideas for tissue engineered MTJ preparation via tissue engineering strategies for damaged MTJ repair and regeneration.