联合Wolbachia和射线技术（IIT/SIT）是一种安全、有效和特异性强的新型蚊虫控制技术。绝育雄虫的适合度，包括飞行能力，生存力和交配竞争力，是影响IIT/SIT蚊虫控制效率主要因素之一。射线辐照会降低绝育雄蚊适合度，但其作用机制仍不清楚。活性氧（ROS）是机体在遭受各种有害刺激时产生的高活性含氧代谢产物，可以破坏DNA或细胞结构，造成机体氧化损伤。同时，ROS含量的升高也会改变肠道微生物的种类和丰度，从而对宿主的生理功能造成重要影响。研究表明辐照会增加昆虫体内ROS水平。因此，申请者拟以白纹伊蚊为研究对象，从ROS水平改变对宿主的氧化损伤和肠道微生物的影响来探讨辐照降低蚊虫宿主适合度潜在作用机制。本研究结果将有助于阐明辐照降低蚊虫适合度的作用机制，为进一步优化蚊虫饲料配方或者辐照方法来提高绝育雄蚊的竞争力，从而提高蚊虫IIT/SIT控制效率，提供理论基础和科学依据。

Combined Wolbachia with irradiation is considered to be a safe, effective and species-specific technique for mosquito population suppression. The sterilized males’s fitness, including the flight ability, longevity and mating competitiveness, is one of the main factors affect the efficiency of IIT/SIT for mosquito control. Fitness reduction of males is observed after irradiation, but the mechanism remains unclear. ROS are the highly reactive oxygenated metabolites and produced when hosts are subjected to various harmful stimulis. ROS can destroy DNA or cellular structures and cause the oxidative damage to hosts. Meanwhile, the increased ROS level would also change the diversity and abundance of the gut bacterium to have influence on hosts’ physiological functions. Moreover, studies have indicated that irradiation would increase the ROS levels in insects. In this study, Aedes albopictus is selected as the research object to explore the potential mechanisms of ROS induced by X-ray irradiation on the fitness reduction of mosquito from the oxidative damage and gut bacterium shifting. The results will help to clarify the mechanisms on fitness reduction of Ae. albopictus caused by irradiation and provide a theoretical basis for further optimizing the mosquito diet formula or irradiation method to improve the fitness of sterilized male mosquitoes, thereby improving the efficiency of IIT/SIT for mosquito conrtol.