Gemykibivirus为Genomoviridae科最新命名的一个病毒属，属于单链环状DNA病毒，该病毒基因组小、变异迅速、重组率高，在多种动物和环境样本中被检测到。而相关前期研究结果显示该病毒可能与人肺炎和脑炎相关，并且其他研究也证明该病毒可能对人进行潜在感染，并会造成较为严重的相关疾病，但是目前，关于该病毒的病原特征尚不清楚。本项目将针对Gemykibivirus这一潜在的新型病原，研究它在人群中抗体阳性率并分析其与临床疾病之间的关联，进一步通过反向遗传学手段获得病毒毒株，探寻这一病毒的感染、复制能力，最后，建立该病毒的小鼠感染动物模型，评估该病毒对哺乳动物的致病性，从而全面解析Gemykibivirus流行病学特点以及生物学特性，为揭示该病毒与人类疾病的关系奠定基础，也为该病毒的流行传播风险评估提供理论支持。

Gemykibivirus, belongs to single-stranded DNA virus, is one of the most recently established genus in the family of Genomoviridae. Though small genome size, rapid mutation and high recombination rate, this virus had been discovered in various animal and environmental samples. The results of previous studies showed that this virus may be associated with human pneumonia and encephalitis, as other relevant studies have also proved. However, at present, the pathogenic characteristics of this virus are still unclear. In this study, the antibody positive rate of Gemykibivirus was calculated by ELISA, and the potential relationship with clinical diseases was demonstrated by improved viral metagenomics technique. Moreover, Gemykibivirus was rescued by reverse genetics for the assessment of infection and replication in different cells. Finally, a mammalian model of infection was established to evaluate the pathogenicity of this virus. The epidemiological and biological characteristics of Gemykibivirus were comprehensively analyzed, which laid a foundation for revealing the relationship between Gemykibivirus and human diseases, and also provided theoretical support for the risk assessment of epidemic transmission of this virus.