生物磁共振波谱分析中的原子标记和非标记方法研究

探测生物体系的分子组成、结构、相互作用及其动态过程，认识这些性质与生命过程的内在联系，是生命分析化学的核心内容之一。细胞是生命过程的基本单元，蛋白质是生物功能的执行者和承载者。细胞环境中获取的生物分子特性信息更有助于认识生命过程的本质。在诸多分析方法中，核磁共振能够在原子水平上提供上述性质的定性和定量信息，独具特色，但活细胞的核磁共振分析尚处于孕育和发展之中。本项目拟以活细胞为主要体系，以蛋白质为主要检测对象，以核磁共振波谱为主要手段，开展基于原子标记和免标记的高灵敏、高选择性分析方法和应用研究。目标是建立普适性的生物波谱分析策略、方法和技术，为探索生物功能的本质提供技术手段和基础数据，同时推进核磁共振波谱在活细胞等复杂体系直接分析中的应用。

Determining the properties of chemical composition, molecular structure, interaction and dynamics of biological system, and then exploring the relationship between these properties and biological processes are one of core subjects for life analytical chemistry. Cell is major organ where most biological processes take place and protein is the key subject for functioning or to bearing the function. Among all analytical techniques, nuclear magnetic resonance (NMR) spectroscopy can provide those properties at atomic resolution. Although application of NMR for in-cell protein study is in its infant, this area has attracted more and more attention. This proposal aims to develop in-cell NMR method in combination with selective atomic labeling and non-labeled strategy for protein interaction and dynamic studies.