基于5-HT/p11/SMARCA3信号通路以慢病毒沉默p11探讨电针对抑郁大鼠海马突触可塑性的作用机制

近年研究发现，小分子蛋白p11能够调节5-HTRs在细胞膜上的表达，促进突触再生，通过5-HT/p11/SMARCA3信号通路发挥抗抑郁作用。我们前期工作表明，电针治疗抑郁症安全有效，其作用与调节海马突触可塑性及神经递质有关,而鉴于5-HT/p11/SMARCA3信号通路在抗抑郁机制中具有重要作用，因此，我们提出“电针对抑郁大鼠海马突触可塑性影响的具体途径是通过5-HT/p11/SMARCA3信号通路实现”的假说。本研究拟采用慢性不可预见性应激（CUMS）结合孤养的抑郁模型大鼠为研究对象，以慢病毒沉默海马p11的表达，借助电生理、RT-PCR、Western Blot、电镜等检测技术，探讨电针治疗抑郁症的作用机制是否通过干预5-HT/p11/SMARCA3信号通路对海马突触可塑性产生影响，从而揭示电针抗抑郁的作用途径和机制，为电针治疗抑郁症的机理阐释提供新的实验依据。

In recent years, it has been found that small molecule protein P11 can regulate the expression of 5-HTRs on the cell membrane, promote the regeneration of synapse, and play a role in anti-Depression through the 5-HT/p11/SMARCA3 signaling pathway. Our previous work showed that electroacupuncture treating Depression is safe and effective, the effect may from the regulation of hippocampal synaptic plasticity and neurotransmitters, and the 5-HT/p11/SMARCA3 signal pathway has the key function in anti-Depression. Therefore, we propose that the effect of electroacupuncture on synaptic plasticity in hippocampus of Depression rats by the 5-HT/p11/SMARCA3 signaling pathway. This study intends to use the chronic unpredicted mild stress (CUMS) with solitary condition model rats, with lentivirus silence the expression of P11 in hippocampal, have the aid of the electrophysiology, RT-PCR, Western Blot, electron microscopy, to explore the mechanism of electroacupuncture on hippocampal synaptic plasticity in treating Depression is whether through the 5-HT/p11 /SMARCA3 signaling pathway or not. Thus to reveal the way and mechanism of electroacupuncture in the treatment of Depression, and provide new experimental evidences for it.