基于电感耦合等离子体质谱技术的OH-PBDEs检测新方法及其在备孕女性体内PBDEs及OH-PBDEs暴露监测中的应用研究

羟基多溴二苯醚（OH-PBDEs）是多溴二苯醚（PBDEs）的羟基化产物，主要来源为海洋环境天然形成或生物体内代谢产生。OH-PBDEs对生物体的毒性效应已被证实要高于PBDEs。研究表明在孕妇和胎儿体内均可能检出OH-PBDEs和PBDEs，并可能会对其健康产生负面效应。我国作为PBDEs生产使用大国，相关环境污染与健康的研究数据仍十分匮乏。随着对PBDEs的禁用，十溴联苯醚（BDE-209）逐步成为我国环境中比重最大的单体，对其分析检测不容忽视。本研究首次提出应用Online SPE-HPLC-ICP-MS方法对有机溴代污染物进行分析检测，在提高高溴代单体测量能力的同时实现对PBDEs和OH-PBDEs的同时分析，应用该方法分析沿海城市天津的备孕女性血清中溴代污染物的赋存浓度，为其暴露监测提供技术和数据支持，同时，还可结合当地代表性海产品中OH-PBDEs的污染特征进行初步源解析。

Polybrominated diphenyl ethers (PBDEs) were a group of widely used flame retardants which have been banned due to their environmental persistence and toxicity. However, decabromodiphenyl ether (BDE-209) is still in use in China and has become one of the most abundant analogue in environment. The hydroxylation of PBDEs has been confirmed as an important metabolic pathway. The metabolites (OH-PBDEs) were considered as contaminants with higher toxicity than PBDEs. The occurrence of PBDEs and OH-PBDEs in pregnant woman and neonate has been confirmed by several researches and the potential healthy damage aroused concerns. As one of the largest producer and user, limited information of this topic can be obtained in China. This research proposed a Online SPE-HPLC-ICP-MS method which could simultaneously analyze PBDEs and OH-PBDEs without derivation of OH-PBDEs. Meanwhile, this method could avoid the degradation of BDE-209 during analysis process. The ionization efficiency in ICP was a satisfaction for PBDEs analysis. The simultaneous determination of PBDEs and OH-PBDEs simplified the pretreatment procedure and made the best use of limited serum samples. The developed method was applied for the analysis of serum sample from pre-pregnant woman in Tianjin City which is a coastal city. The results could provide data for the exposure estimation. Besides, the comparison between pollution profiles in serum and local sea food could be an approach for preliminary investigation and parsing of pollutants sources.