家蚕转录因子BmPOUM2基因可变剪接的调控机理

项目以调控家蚕翅原基变态发育的转录因子BmPOUM2为研究对象，采用分子生物学、细胞生物学、发育生物学和转录组学等方法，研究BmPOUM2在不同发育时期产生不同剪接形式的可变剪接机理。研究内容包括：1）BmPOUM2两种剪接形式的表达与功能分析；2）BmPOUM2剪接调控元件的鉴定；3）BmPOUM2剪接调控因子的鉴定；4）BmPOUM2剪接调控因子的功能分析。研究将有助于阐释家蚕翅变态发育的机理，也可为动物组织器官的发育和再生机理的阐明提供重要的线索，并有可能发展相关的剪接调控元件或剪接调控因子为调控昆虫生长发育的新靶标位点或靶标分子。

This project will study the alternative splicing mechanism of the transcription factor BmPOUM2 that be involved in the metamorphosis of wing disc and produced the different isoform during the different stages in the domesticated silkworm by using the approaches of molecular biology, cellular biology,developmental biology, transcriptomics,and biotechnology. The research contents include: 1) expression and functional analysis of BmPOUM2 isoforms; 2) identification of the splicing regulatory elements of BmPOUM2; 3) identification of the splicing regulatory factors of BmPOUM2; 4) functional analysis of the splicing regulatory factors. The study will be important for the explanation of the molecular mechanisms of wing disc metamorphosis. It can also provide important clues to the clarify for the development and regeneration mechanism of animal tissues and organs, and may develop the relative splicing regulatory elements or splicing regulatory factors to the new target sites or target molecules which regulate the insect development.