HPV16 DNA甲基化与维吾尔族妇女宫颈癌前病变相关性研究

高危型HPV持续性感染是宫颈癌发生的主要原因，大部分感染可自行清除，仅有10%左右的个体发生进展，但是关于HPV感染究竟是自行清除还是会进一步进展，目前仍然缺少明确的标志物。新疆维族妇女人群具有较高的宫颈癌发病率和较低的HPV感染率，提示HPV阳性的维族妇女更易发生宫颈癌变，在宫颈癌的发生和进展方面具有族群的特异性。HPV16 DNA特定位点CpG的甲基化会调控病毒癌基因的转录和表达，从而影响病毒对宿主细胞的转化。本课题拟对HPV16阳性且宫颈细胞学正常或慢性炎症的维族妇女是否会发生宫颈癌前病变进行前瞻性研究，利用甲基化焦磷酸测序、HPV基因芯片分型和宫颈细胞学诊断技术，探讨HPV16L1区和URR区甲基化状态与维族妇女HPV的持续感染、宫颈细胞病变进展的相关性。研究可能为HPV阳性的维族妇女中高风险个体的筛选分流提供新的标志物，为维族妇女宫颈癌的早期防治提供新的思路。

Persistent infection of high-risk HPV is the main cause of cervical cancer. Most infections clear by host itself, only about 10% will progress. But there still absence good biomarker to distinguish short-live infection and further progress. The population of Xinjiang Uyghur women has a higher incidence of cervical cancer and a lower infection rate of HPV. It indicates that Uyghur women are more likely to prompt cervical lesions in HPV-positive crowd. The methylation change of anchor CpG site will regulate the transcription and expression of viral oncogene, thus affecting the conversion of the host cell. The project is a prospectively study to the Uyghur women with HPV16 infection and normal cervical cytology. The techniques of Methylation pyrosequencing , HPV genotyping gene chips and cervical cytology diagnostic will be used. The correlation between HPV persistent infection plus cervical cell changes and methylation status of HPV16L1 / URR in Uyghur women will be explored in this study. The research may provide a new biomarkers to distinguish the high-risk individuals in Uyghur with HPV, and provide new ideas for early prevention and treatment of cervical cancer in Uyghur women.