拟南芥ABC转运蛋白SAA1参与芥子酸及其衍生物转运的功能鉴定及调控机理研究

苯丙烷类代谢物质在植物细胞分化、木质发生、色素形成以及生物和非生物胁迫等方面有着广泛的生理功能，一直是次生代谢研究领域所关注的热点。尽管人们对于该类物质代谢途径有了一定的了解，但对于这些物质如何合成、转运和积累，仍然需要作进一步的深入研究。我们通过酵母表达系统筛选得到一个拟南芥ABC转运蛋白SAA1具有转运芥子酸的活性。有趣的是，在含有1.5mM的芥子酸的MS培养基上，拟南芥SAA1的T-DNA插入突变体saa1的生长明显受到了抑制。本课题利用酵母AD1-9和拟南芥saa1突变体并借助于生物化学、细胞生物学、遗传学和分子生物学等多学科技术，探讨SAA1参与芥子酸及其衍生物等苯丙烷类物质转运和调控的机理。该项目的实施，不仅解析SAA1参与植物芥子酸以及衍生物等苯丙烷类代谢物质的运输过程，而且也为揭示芥子酸酯是否在种子萌发和生长发育以及环境适应等方面具有调节作用提供理论依据。

The physiological role of phenylpropanoids is the hot topic in field of research in secondary metabolism, such as plant cell differentiation, lignification, pigment formation and biotic and abiotic stress, etc. People have already certain knowledge in phenylpropanoid metabolism. However, very little is known how these metabolites are transported from generating tissues to specific functional pools in plant growth regulation or in response to stresses. Here we selected Sinapic acid-assaciated ATP-Binding Cassette transporter genes (SAA), in the ABC transporter family. SAA1 regulates sinapic acid transportation by yeast screening system. Interestingly, the growth of SAA1 T-DNA insertion mutants (saa1) was inhibited in MS medium with 1.5 mM sinapic acid compared with wild-type. These results suggested that SAA1 propably is involved in phenylpropanoid metabolisms and transportation, such as sinapic acid or its esters, etc. In this research, using AD1-9 yeast mutant and saa1 mutants in Arabidopsis, we will study on the mechanism of SAA1-involved in transportation and regulation of phenylpropanoid metabolisms. By the implementation of this project, this not only will illustrate that SAA1 propably involves in phenylpropanoids transportation, but also provide important theoretical evidence for revealing the role of sinapic acid ester in regulation of seed germination and plant growth and development, resistance to environmental stress.