游离移植脂肪存活与脂肪细胞动态分化调控机制的相关性研究

自体脂肪游离移植后较高的吸收率限制了其临床应用。如何在体内实现对移植后脂肪组织存活机制的调控，是保障其临床应用的关键。我们已经证明体外环境下脂肪细胞通过去分化-再分化变化增强细胞对缺氧环境的耐受力并存活，那么，体内环境中脂肪细胞是否存在动态分化调控机制？前期研究发现，游离脂肪移植后湿重曲线晚期出现细微上升的变化趋势，移植物早期释放脂质，中晚期出现大量幼稚脂肪细胞的分化。据此，我们推测：体内环境中存在脂肪细胞去分化-再分化的动态变化，并且游离移植脂肪存活与脂肪细胞动态分化调控机制密切相关。本项目拟建立扩张器压迫大鼠脂肪垫缺血模型和绿色荧光蛋白标记游离脂肪移植模型，采用定期扩张技术、基因检测手段、病理分析技术等，重点研究体内缺血环境脂肪细胞的分化调控能力，及其与游离移植脂肪存活的密切相关性，阐明移植脂肪细胞存活的内在机制，为提高组织存活率，促进脂肪游离移植的广泛临床应用打下坚实的理论基础。

A relatively high absorptivity limits the clinical application after free autologous fat grafting. The way how to regulate the mechanism of adipose tissue after transplantation in vivo is the key to ensure its clinical application. It is already approved that adipose has enhanced its tolerance against anoxic condition and been able to survive in vitro through Dedifferentiation-Redifferentiation, then, if there is a mechanism of dynamic differentiated regulation on adipocytes in vivo? At early study, weight curve appeared a tendency as slow increase after the grafting late time, the transplants released lipide in early stage, and abundant immature adipocytes re-differentiated in mid-late stage. Thus, we presume that: there is a dynamic change of adipose Dedifferentiation-Redifferentiation in vivo, and free transplanted fat survival is closely related to the mechanism of dynamic differentiated regulation on adipocytes. This article drafts to create a dilator-compressed rat's fat pad ischemia model and a GFP marker free fat grafting model，introducing periodical extension technique、gene test method、pathological analysis technology etc, to study the ability of differentiated regulation on adipocytes under anoxia in vivo, and its correlation with free transplanted fat survival emphatically, clarifying the internal mechanism of free transplanted fat survival, laying a solid theoretical foundation for improving tissue survival ratio, promoting a widely application of fat freeing and transplantation clinically.