游离脂肪是最理想的软组织填充剂之一，移植后的脂肪再生被认为是决定其转归的关键。课题组前期研究发现：通过机械力可诱导组织内成熟脂肪细胞出现脂滴分解、细胞骨架改变实现去分化转变，且伴有脂肪细胞棕色化表现，此转变可显著提高脂肪移植后的保留率。机械力作用下，脂肪细胞骨架蛋白integrin β1显著上调，但其如何诱导白色脂肪棕色化的具体机制尚不明确。本研究拟采用组织移植技术、细胞生物学技术、蛋白生化技术、病理检测等技术，观察机械力作用下integrin β1/ERK通道在诱导白色脂肪棕色化过程中的作用，及其对游离脂肪移植后脂肪再生的影响。本研究将进一步揭示机械力诱导白色脂肪棕色化过程中的信号通路，探讨白色脂肪棕色化预处理后提高游离脂肪移植后脂肪再生的潜在价值及可能机制。

Autologous fat transplantation is a popular technique for tissue augmentation. Fat regeneration after transplantation is considered to be the key to its retention rate. Previous studies have found that mechanical force can induce lipid droplet decomposition and cytoskeleton changes in mature adipocytes to achieve dedifferentiation. During this process, browning of adipocytes was also observed. Adipocyte browning can significantly improve the retention rate after fat transplantation. Integrin beta-1, a cytoskeleton protein of adipocytes, is significantly up-regulated by mechanical forces, but the specific mechanism of how it induces browning of white fat is unclear. In this study, we used tissue transplantation, cell biology, protein biochemistry and pathological examination to observe the role of integrin beta 1/ERK pathway in inducing browning of white fat and its effect on fat regeneration after free fat transplantation. This study will further reveal the signaling pathways in the process of browning of white fat induced by mechanical force, and explore the potential value and possible mechanism of browning of white fat to improve fat regeneration after free fat transplantation.