湿证是慢性胃炎（CG）常见证候之一。本课题组前期研究检测了CG湿证舌苔中差异蛋白和CG腻苔中差异代谢物，并发现CG舌象信息客观参数、舌苔免疫指标与胃镜特征之间存在相关性。基于此，本项目应用气-质联用、液-质联用等代谢组学技术检测CG湿证、CG非湿证、亚健康湿证和正常人舌苔中代谢物：①通过多元变量统计等数据处理及生物信息学方法对舌苔代谢物进行定量、定性分析，筛选CG湿证的舌苔代谢标志物，鉴定代谢标志物的化学结构及功能，阐释CG湿证的代谢通路等发生机制；②运用典型相关分析探讨CG湿证舌苔代谢标志物与胃镜特征的相关性，并基于舌苔代谢标志物及胃镜特征，通过支持向量机、决策树、随机森林等方法建立CG湿证诊断模型，最后对模型进行临床验证、评价及完善。以期为湿证的物质基础及发生机制提供代谢组学方面的实验依据；并通过探索用无创的舌苔代谢组学检测辅助胃镜检查，为CG病证诊断、疗效评价及预后监控提供研究基础。

Dampness syndrome is one of the common Traditional Chinese Medicine(TCM) syndromes of chronic gastritis(CG). In our previous researches we had found some differential protein molecules in tongue coating of patients with dampness syndrome of CG, some differential metabolic molecules in greasy tongue coating of patients with CG and the association between tongue information parameters, tongue coating immune index of patients and gastroscopy characteristics. Based on this foundation, we will use metabolomics methods based on the application of gas chromatography-mass spectrometry technology and liquid chromatography-mass spectrometry technology to detect metabolites in tongue coating of patients with dampness syndrome and non-dampness syndrome of CG, dampness syndrome of sub-health and normal: ①make a quantitative and qualitative analysis of tongue coating metabolites by means of multivariate data processing and bioinformatics methods, screening tongue coating metabolic markers CG dampness, identify the chemical structure and function of metabolic markers, and explain the metabolic pathway of dampness syndrome of CG; ②use the canonical correlation analysis to investigate the correlation between tongue coating metabolism markers and gastroscopic features of dampness syndrome of CG, through the support vector machine, decision tree, random forest and other methods to establish diagnostic model of dampness syndrome of CG based on tongue coating metabolite and endoscopic characteristics, and finally verify, evaluate and improve the model. This research will provide the experimental basis for the material basis and mechanism of dampness syndrome, and also provide the basis for the diagnosis, evaluation of therapeutic effect and prognosis of CG through exploring the use of noninvasive tongue coating metabonomics in the detection of assisted gastroscopy.