铁杉属三种我国特有植物次级代谢产物及其生物活性研究

我国特有植物是中草药的重要组成部分，也是比较独特的部分，因为特有植物往往具有特殊的次级代谢产物。本课题旨在系统的研究铁杉属中长苞铁杉、铁杉和南方铁杉这三种我国特有珍稀植物的次级代谢产物及其生物活性，重点研究木脂素类和三萜类化合物的结构特征、生物活性和构效关系，阐明其活性成分并确定一个先导化合物。拟通过中压硅胶柱色谱、MCI、Sephadex LH-20、制备HPLC等现代色谱技术对这三种我国特有植物进行系统的次级代谢产物分离纯化。通过MS、NMR和X-单晶衍射等现代波谱学方法对所分得的化合物进行结构鉴定。对纯化得到的木脂素类和三萜类化合物进行体外细胞毒和杀蚜虫活性筛选，阐明活性成分，分析构效关系。这将为我国特有植物资源的合理开发和利用提供科学依据，也将从植物化学分类学的角度有助于铁杉属植物的系统分类。本课题拟分离鉴定80余个化合物，发现5-20个新化合物，发表SCI论文4篇，申请专利1项。

Endemic plants are important and unique components of Chinese herbal medicine in our country, because endemic plants often produce special secondary metabolites. This project is aim to study on the secondary metabolites and their bioactivities of three Chinese endemic rare plants of Tsuga, including Tsuga longibracteata Cheng, Tsuga chinensis (Franch.) Pritz., and Tsuga chinensis (Franch.) Pritz. var. tchekiangensis (Flous) Cheng et L. K. Fu. We focus on the chemical structure characteristics, biological activity and structure-activity relationship of lignans and triterpenoids，and then clarify the active ingredient and find a lead compound. The isolation and purification was using chromatographic methods such as medium pressure silica gel, MCI, Sephadex LH-20 column chromatographic technology, and preparative HPLC. The structures of the secondary metabolites will be elucidated on the basis of spectroscopic techniques including MS, NMR, and X-ray diffraction. These compounds of lignans and triterpenoids will be evaluated for their cytotoxicity and insecticidal activities. Then, the biological activity constituents and structure-activity relationship were being research. This provides the scientific evidences for the reasonable application and development of Chinese endemic plant resources, for the determination of the classification of Tsuga plants from the chemotaxonomy aspect. This project were plan to isolate and purify more than eighty compounds, including five to twenty new compounds, to publish four SCI articles of the phytochemistry research fields, and to apply for a patent.