老鹰茶中吡喃酮并黄烷醇类成分的发现及其抗肿瘤作用机制研究

老鹰茶是西南地区特色的药食同源植物，其药化和药理还需深入研究以阐明其药效物质基础。前期我们从老鹰茶中发现有很好的抗肿瘤细胞毒活性的吡喃酮并黄烷醇类新骨架化合物。本课题旨在从老鹰茶中定向获得多个此类新颖小分子，并探讨其抗肿瘤作用机制。本项目将以UPLC-DAD-MS/MS为指导，结合色谱和质谱特征定向快速获得此类成分，通过NMR等波谱学方法对其进行结构鉴定。通过流式细胞术研究化合物对细胞周期、早期凋亡的影响，通过WB技术，研究其信号通路，阐明其抗肿瘤作用机制。

Hawk tea is a medicinal and edible plant distribution in the southwest of China. The work of its pharmacodynamics material basis still need further research by medicinal chemistry and pharmacology methods. In a previous investigation on Hawk tea we had isolated two novel pyranone[2,3-g]flavan-3-ol derivatives with excellent anti-tumor cytotoxic activity. So, this project is aim to study on the chemical structure and anti-tumor mechanism of pyranone[2,3-g]flavan-3-ol derivatives from Hawk tea. The isolation and purification will be guide by UPLC-DAD-MS/MS, chromatography and spectroscopy characteristics to obtain the pyranone[2,3-g]flavan-3-ol derivatives, targeting and quickly. The structures of these compounds will be elucidated on the basis of spectroscopic techniques including HR-MS and NMR. Owe to the flexibility of long fatly chain. The anti-tumor activities mechanism of these compounds will be researched. Flow cytometry was employed to detect the effects of the compounds on cell cycle and early apoptosis. Expression levels of apoptosis-related proteins were determined by western blot assay.