田间劣变导致的种子活力降低已成为许多热带和亚热带大豆产区的共性问题，严重影响了春大豆种子质量乃至大豆产业的发展。目前对春大豆种子田间劣变抗性的调控机制尤其是分子调控机制尚缺乏系统研究。项目组前期研究表明大豆Homeobox类转录因子基因GmSBH1参与春大豆种子田间劣变过程，但作为转录因子，高温高湿下GmSBH1在调控春大豆种子田间劣变抗性过程中是如何发挥作用的尚不清楚。本项目拟从高温高湿下调控GmSBH1转录的上游蛋白及作用方式、高温高湿下与GmSBH1互作的关键蛋白及互作方式、GmSBH1及其关键互作蛋白基因共调控种子田间劣变抗性的功能验证、高温高湿下受GmSBH1调控且与种子田间劣变抗性相关的下游基因4方面为切入点，揭示高温高湿下GmSBH1调控春大豆种子田间劣变抗性的分子机制。为构建春大豆种子田间劣变抗性的分子调控网络和开展春大豆种子田间劣变抗性品种分子设计育种奠定理论基础。

The seed vigor decrease due to the pre-harvest seed deterioration frequently occurs in many tropical and sub-tropical soybean production areas around world，which seriously impact the seed quality as well as the soybean industry. The molecular regulation mechanism of the pre-harvest seed deterioration resistance is poorly understand. It has been shown that GmSBH1 related to the pre-harvest seed deterioration, but the regulation roles of GmSBH1, a homeobox transcription factor, was not fully studied. The objective of this project is to reveal the molecular regulation mechanism of GmSBH1 on the pre-harvest seed deterioration resistance of spring soybean from three aspects, first is to analyze the upstream protein that regulated GmSBH1 transcription under the high temperature and humidity, second is to identify the key proteins that regulated the pre-harvest seed deterioration resistance under high temperature and humidity by interaction with GmSBH1, third is to identify the function of GmSBH1 and the interactions protein gene that regulated the pre-harvest seed deterioration resistance under high temperature and humidity, and fourth is to study the downstream genes that regulated by GmSBH1 and related to the pre- harvest seed deterioration resistance under high temperature and humidity. This results of this project expected to facilitate the construction of the molecular regulation mechanism network for the pre-harvest seed deterioration resistance under high temperature and humidity, to implement molecular design breeding of new spring soybean germplasm with the pre-harvest seed deterioration resistance.