Transcriptomic and photosynthetic responses to grafting of the Nod1 gene in nodulated and non-nodulated soybeans.
Transcriptomic and photosynthetic responses to grafting of the Nod1 gene in nodulated and non-nodulated soybeans.
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结瘤和非结瘤大豆中 Nod1 基因嫁接的转录组和光合反应
DOI：
10.1093/g3journal/jkab209
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发表时间：
2021-09-06
期刊：
影响因子：
--
通讯作者：
Zhao T
中科院分区：
文献类型：
--
作者：
He Q;Xiang S;Wang W;Shu Y;Li Z;Wang S;Chen L;Yang X;Zhao T
豆科植物与根瘤菌形成共生关系，将氮气转化为氨，结瘤状态会影响植物的发育，包括光合作用。然而，碳、氮代谢过程中固氮与光合作用的关系尚不清楚。本研究利用一个自发的非结瘤大豆突变体，通过嫁接来解开大豆结瘤和光合作用的调节。遗传和基因定位结果表明，该非结瘤突变体是由一个与已报道的RJ1基因座位重叠的隐性基因控制的，与正常结瘤植物的序列比较，可能是一个新的RJ1等位基因，第4外显子缺失1个 碱基。根据嫁接结果，大豆结瘤明显是由根部决定的，而不是幼苗。此外，非根瘤接穗嫁接节根，提高了植株的氮素含量和相关代谢酶活性，提高了光合作用能力。将节状接穗嫁接到非结瘤根上则得到相反的结果。通过对嫁接处理的转录组分析，共鉴定出853个叶片差异表达基因(Deg)和1874个根差异表达基因(Deg)。我们在叶片中识别出285个差异基因本体论术语和57个差异路径术语，在根中识别出856个差异基因本体论术语和207个差异路径术语。选择了20个在翻译水平上相互作用的基因，并用Q-PCR对转录组分析结果进行了验证。这些结果表明，与结瘤相关的NOD等位基因增加了非结瘤植物的氮素含量，影响了参与氮代谢的酶，导致激素水平的变化，进一步调节了光合作用和碳代谢。
Legume plants form symbiotic relationships with rhizobia to convert N2 into ammonia, and the nodulation status can affect plant development including photosynthesis. However, the relationship between nitrogen fixation and photosynthesis during carbon and nitrogen metabolism remains unclear. This study was undertaken to unravel regulation of nodulation and photosynthesis using a spontaneous nonnodulated soybean mutant by grafting. The results of inheritance and gene mapping showed that the nonnodulated mutant was controlled by a recessive gene overlapped with the reported rj1 locus, and might be a new rj1 allele with 1 bp deletion in the fourth exon in comparison to the sequence of normal nodulation plants. According to grafting results, soybean nodulation is obviously determined by the roots, not the seedlings. Moreover, nitrogen content along with related metabolic enzyme activity, and photosynthetic capacity were enhanced by nonnodulated scions grafted with nodulated roots. Contrary results were obtained for nodulated scions grafted with nonnodulated roots. A total of 853 differentially expressed genes (DEGs) in the leaves and 1874 in the roots were identified by transcriptome analyses of the grafting treatments. We identified 285 differential gene ontology (GO) terms and 57 differential pathway terms identified in the leaves, while 856 differential GO terms and 207 differential pathway terms in the roots. Twenty DEGs interacting at translation level were selected, and the results of transcriptome analyses were verified by q-PCR. These findings indicated that the nodulation-related Nod allelic gene increases the nitrogen content of nonnodulated plants, which affects the enzymes involved in nitrogen metabolism, leading to changes in hormone levels and further regulation of photosynthesis and carbon metabolism.
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DOI：
10.1093/dnares/dsr033
发表时间：
2011-12
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Kim KH;Kang YJ;Kim DH;Yoon MY;Moon JK;Kim MY;Van K;Lee SH
通讯作者：
Lee SH
影响因子：
16.6
作者：
Jin Y;Liu H;Luo D;Yu N;Dong W;Wang C;Zhang X;Dai H;Yang J;Wang E
通讯作者：
Wang E
影响因子：
16.6
作者：
Madsen, Lene H.;Tirichine, Leila;Jurkiewicz, Anna;Sullivan, John T.;Heckmann, Anne B.;Bek, Anita S.;Ronson, Clive W.;James, Euan K.;Stougaard, Jens
通讯作者：
Stougaard, Jens
影响因子：
2
作者：
He, Qingyuan;Yang, Hongyan;Gai, Junyi
通讯作者：
Gai, Junyi
影响因子：
6.9
作者：
FRANCISCO, PB;AKAO, S
通讯作者：
AKAO, S