类胡萝卜素纳米输送体系作为兼具高稳定性、高生物利用率的食品载体，引起国内外广泛关注。以往研究主要集中于其物理化学稳定性及体外消化特性，而由受体介导的肠道吸收机制研究尚未见报道。本项目通过优化β-胡萝卜素纳米输送系统体外消化模型，建立Caco-2细胞SR-BI敲低单层模型，研究SR-BI介导脂肪酸调节β-胡萝卜素跨膜转运的作用机理；从体外生化、细胞转运的角度探明β-胡萝卜素/脂肪酸/SR-BI三者之相互作用与体系生物利用率之间的相关性，为提升β-胡萝卜素纳米输送体系在食品营养领域的应用提供科学依据及理论基础。

Carotenoids nano delivery systems have become popular for their sound stability and high bioavailability. The previous study focused on their physical and chemical stability and in vitro digestion; however the mechanisms of their transporter-mediated intestinal absorption have been rarely reported. This proposed research will combine the studies of the in vitro digestion model and SR-BI knock-down Caco-2 single-layer model , (a) systemically investigate the effect of fatty acids on β-Carotene intestinal absorption based on transporter SR-BI; and (b) test the correlation of β-carotene/fatty acids/SR-BI interaction and systematic bioavailability. Results of this research will help reveal the molecular network of SR-BI-mediated intestinal absorption of β-Carotene nano delivery systems, and provide theoretical and practical basis for their applications in food industry.