针对目前定量蛋白质组学中具有重要生物学意义的低丰度蛋白质难于富集的问题，本项目拟通过计算机辅助手段，筛选出肿瘤标记物蛋白质 C-末端肽段作为模板分子，通过聚醚砜相反转自组装的方式制备分子印迹材料，并用于血清中肿瘤标记物的选择性富集。通过聚醚砜相反转热力学和动力学研究、C-末端与聚醚砜链相互作用研究以及印迹材料吸附动力学研究，探索分子印迹材料的制备以及识别机理。此外，基于可逆加成-断裂链转移聚合，制备可与聚醚砜共混自组装的活性/可控聚合物链段，发展新型的印迹聚合物功能改性方法，提高印迹材料对肿瘤标记物蛋白质的选择性。本项目研究内容不仅对发展新型蛋白质识别材料具有重要的科学意义，而且在蛋白质组学研究中低丰度蛋白富集方面有很强的应用价值。

The enrichment of low abundance proteins is one of the challenges for the quantitative proteomics study. In this project, C-terminal peptides of the tumor marker proteins, will be selected as the template with the computer assistance. The protein C-terminal imprinted materials will be prepared by the phase inversion self-assembly of polyethersulfone (PES) for the selective recognition of the tumor marker proteins in the serum. The mechanism investigation on the fabrication and the recognition of the imprinted materials will be studied by the thermodynamics and kinetics of the PES phase inversion during the particle formation, the interaction between the PES and C-terminal peptides, and the kinetics model of adsorption. In addition, controlled/living polymer chain will be prepared by reversible addition-fragmentation chain transfer (RAFT) polymerization for the blending with the PES. This new modification on the imprinted materials would favor the selectivity of the imprinted materials toward the tumor marker proteins. This project not only has significant scientific values for the development of protein imprinted materials, but also would show its promising potentials on the low abundance protein enrichment in the proteomics study.