玉米是中国第一大粮食作物，2013年种植面积达到5.3亿亩。玉米生长期遭遇低温，会引起植株体内发生一系列生理代谢反应，进而影响其产量和品质的建成。ASR基因是近年来从植物中发现的一类基因，在植物生长发育、胁迫应答中发挥重要生理功能。尽管不同植物中已有部分ASR被克隆，但玉米低温下ASR1的研究，国内外尚未见报道。低温胁迫下玉米ASR1基因的表达与ABA信号传导是否存在通路反应，尚不清楚。本研究对玉米低温胁迫响应基因ASR1进行克隆及基因表达特性分析，并对基因功能进行验证研究，从外源ABA诱导ASR1基因表达出发，探明ASR1基因表达与ABA之间信号传导关系。旨在为农业生产上提高玉米抗低温能力奠定基础，也为其它禾本科作物的抗冷研究提供技术方法。

Maize is the first major food crops in China and the acreage reached 5.3 million mu in the 2013.Maize suffered a low temperature in growing season，which will cause a series of physiological and metabolic reactions，thereby affecting the yield and quality built.ASR gene is a class of genes from plants found，which play an important physiological function in the stress response.The study of ASR1 at low temperatures has not been reported.Whe ther the low temperature stress maize ASR1 gene expression and ABA signaling pathway reaction exists in the process is not clear.In this study，maize low temperature stress response genes ASR1 was clonied.Gene expression characteristics analysis and gene function validation were studied between ASR1 gene expression and ABA.The study aims to raise maize on the cold resistance of the basis for agricultural production，but provice technical methods for other gramineous crops chilling.