吲哚族芥子油苷介导拟南芥年龄相关抗性机制的研究

植物发育后期对病原菌抗性急剧增强的现象称为年龄相关抗性（Age-related resistance，ARR）。ARR抗病机制与之前时期大不相同，但具体机制尚知之甚少。前期研究中我们发现催化色氨酸合成吲哚乙醛肟（IAOx）的CYP79B2和CYP79B3双缺失可导致拟南芥无法形成ARR，对IAOx下游三条代谢途径中关键基因突变体进行ARR检测，发现吲哚族芥子油苷（IGS）途径参与ARR过程。结合前人研究我们对IGS参与ARR的机制提出三种假设：1. IGS抑制IAA信号途径，增强抗性；2. IGS被分泌至细胞间隙发挥抗菌作用；3. IGS作为信号分子激活ARR相关反应。我们将以拟南芥相关突变体为材料，利用分子生物学技术验证这3种假设，并进一步通过转录组学和代谢组学手段全面揭示ARR的独特抗病机制。本项目将为ARR研究提供全新思路，针对影响植物质量和产量的关键时期，为植病的防御提供理论基础。

In the later stage of development, older plants display rapidly increased resistance to some pathogens and this form of resistance is called age-related resistance (ARR). At present, it has been proved that the mechanism of ARR is quite different from that in the previous development stage, but little is known about how ARR actually works. In our previous study, we found that loss of function in CYP79B2 and CYP79B3，which catalyze tryptophan to IAOx lead to deficiency of ARR in Arabidopsis. Based on this study, ARR detection was carried out in some of the deficient mutants in metabolic pathways downstream of CYP79B2/3 catalytic product. The study showed that indole glucosinolate metabolism pathway was involved in ARR. Combining our research with previous studies, we established three scientific hypotheses about the possible mechanism of indole glucosinolates involving in ARR. 1. IGS inhibited IAA signaling pathway and enhanced resistance; 2. IGS was secreted into the intercellular space to play an antibacterial role; 3. IGS activated ARR related reactions as a signal molecule. We will take Arabidopsis related mutants as materials, use molecular biology technology to verify these three hypotheses, and further reveal the unique resistance mechanism of ARR by means of transcriptome and metabolomics. This project will provide a new idea for the research of ARR, and provide a theoretical basis for the prevention of plant diseases in the key period affecting plant quality and yield.