哺乳动物正呼肠孤病毒属于呼肠孤病毒科，正呼肠孤病毒属，在哺乳动物中的感染非常普遍。传统认为正呼肠孤病毒感染宿主后引起的病症轻微且症状不明显，但目前有越来越多的研究表明其在多种动物中能引起呼吸道和脑炎等严重的疾病。蝙蝠是多种新发传染疾病的自然宿主，包括埃博拉，亨尼帕和SARS冠状病毒等，但目前世界范围内对来源于蝙蝠的正呼肠孤病毒对人及其他动物的致病性和跨种传播感染的危险性研究还很少。我们先前从蝙蝠粪便和尿液样品中成功分离到6株呼肠孤病毒，基因组序列分析表明这些病毒分属于哺乳动物正呼肠孤病毒血清型1-3型，并且与患病貂、猪以及儿童的分离株遗传进化关系最近。本研究计划通过细胞和动物感染以及血清学中和实验研究不同血清型的蝙蝠正呼肠孤病毒宿主范围和组织嗜性，阐明其对人及其他动物潜在的致病性。研究结果将为蝙蝠源的正呼肠孤病毒的致病性及其跨种传播感染的预防和控制提供理论基础和技术支持。

Mammalian orthoreovirus (MRV), belonging to the Reoviridae, Orthoreovirus, are ubiquitous in all mammalian species. Traditionally, they are associated with mild and clinically unapparent infections, but there is growing evidence of their ability to cause severe respiratory disease and encephalitis in human and other animals. Bats are natural reservoir of some emerging infectious diseases, including Ebola virus, Nipah virus, Hendra virus and SARS coronavirus. However, little is known about the pathogenicity of bat-derived reoviruses to human or other animals and the risk of interspecies transmission. Previously, we isolated six strains of MRV from bat feces and urine samples. These bat MRVs, classified to MRV serotype 1, 2, 3 based on their genome sequences, have high similarity with the isolates from ill mink, piglets and children. This project will investigate the their host range, tissue tropism and serological cross-reactive with other animal or human MRVs, in vitro and in vivo, and evaluate the potential pathogenicity to human and other animals. This study will provide theoretical basis and technical support for future prevention and control of bat MRV infection.