成纤维生长因子23在笼养蛋鸡骨质疏松发生中的作用研究

钙磷代谢紊乱导致的骨质疏松（CLO）是影响当前蛋鸡养殖的一种重要疾病。成纤维生长因子23（FGF23）是2000年发现的一种钙磷代谢调控因子，在Klotho辅助下与FGFRs结合而影响钙磷代谢，FGF23表达异常动物出现骨代谢紊乱症状，但机制不清楚。项目组已证明：破骨细胞和蛋壳腺存在FGFRs和Klotho表达；CLO蛋鸡FGF23水平显著高于健康鸡，提示FGF23在CLO发生中有重要作用。项目拟应用real-time PCR和micro-CT等方法检测健康和CLO蛋鸡FGF23以及血液-骨-肠-肾-骨-蛋壳腺中钙磷代谢相关激素/因子含量或mRNA表达及骨生物学特性的改变；通过细胞培养试验，应用western blot等方法检测FGF23对破骨/成骨细胞分化、骨吸收、骨形成以及两种细胞对话因子表达的影响，最终阐明FGF23在CLO发生中的作用及机制，为生产中CLO的诊断和预防提供理论依据。

Cage layer osteoporosis (CLO), induced by calcium and phosphorus metabolic disturbance, is one of the serious diseases in laying hens. Fibroblast growth factor 23 (FGF23) which was discovered in 2000 has been defined as a hormone regulating calcium and phosphate homeostasis by binding to FGFRs under the help of its coreceptor Klotho in target tissues. Abnormalities of bone mineralization in mice were observed in accociation with FGF23 excess or deficiency, but its mechanism is still unclear. We have previously found that FGFRs and Klotho mRNA were expressed in osteoblasts and uterus of laying hen. The concentration of serum FGF23 in CLO hens was obviously higher than that of wild type, suggesting that FGF23 may play a vital role in CLO. The present study aims to determine the level or mRNA expression of FGF23, hormones or factors related to calcium and phospate metabolism in serum, duodenum, kidney, bone and uterus, as well as the change of bone bionomics in CLO and wild type laying hens by real-time PCR and micro-CT. Meanwhile, effect of FGF23 on differentiation of osteoblasts and osteoclasts, bone resorption and formation, communicated factors expression between osteoblast and osteoclast will be approached through real-time PCR and western blot in vitro. Finally, the regulative mechanisms of FGF23 in CLO will be elucidated in order to provide a theoretical foundation for CLO diagnosis and prevention in laying hens industry.