胶质瘤干细胞的放射抗拒性是放疗后胶质瘤未控和复发根源。既往研究多以肿瘤细胞为对象，鲜见分子水平胶质瘤干细胞放射敏感性及机制的研究。特别是在体研究中，有关胶质瘤干细胞特异性的标记及有效干预靶点方面都少有研究。基于此，我们在前期已合成胶质瘤靶向探针的基础上，进一步构建特异识别胶质瘤干细胞探针；同时，基于AEG-1基因在胶质瘤组织中的特异性高表达及其在复杂细胞信号通路网络中的关键地位，依据我们之前对信号通路与肿瘤放射敏感性关系的研究，推测AEG-1基因可能与肿瘤干细胞放射敏感性有关并受多个信号通路的调节。本研究拟用聚酰胺 (Polyamidoamine, PAMAM)建立高分子纳米粒胶质瘤干细胞靶向探针，并以其为活体观测及干预载体，利用RNAi等分子生物学技术，探寻AEG-1基因在胶质瘤干细胞放疗抵抗机制中的作用及其分子信号转导机制，为提高临床胶质瘤放疗预后提供实验依据。

Radioresistance of glioma stem cells(GSC）is the root of persistent or neurospongioma. Previous studies have spend much attention in tumor cells but rared reports on radiosensitivity of the tumor stem cells and its mechanism maintaining. Especially in vivo, nather about the GSC specific markers or the effective therapeutic target has rarely been reported. On the basis of the previous studies about the targeting nanoprobe of glioma,we want to make a further research on a new probe for GSC。Further more，linking the important role of AEG-1 gene in glioma biology characteristics and its key position in the complexnetwork of cell signaling pathways with the relationship between signaling pathways and radiosensitivity ofthe tumor cells,we propose a bold hypothesis that AEG-1 represented a viable potential target for the radiotherapy of glioma, and subjected to multiple signaling pathways.In this study, ,we want to designe and synthesize a novel polymer nanoprobe targeting of GSTC,and useing the nanopobe as observation methods in vivo, we study to explore the effect of RNA interference （RNAi）-mediated AEG-1 gene silencing the radiosensitivity of GSC and investigate possible mechanisms,provide laboratory data to improve the prognosis of glioma.