子宫腺肌病已逐渐成为妇科常见病、疑难病，其痛经发生率高达77.8%，严重影响患者生存质量。子宫腺肌病痛经与子宫平滑肌细胞受ER与BKca、TRPV-1离子通道异常调控而过度收缩有关。我们的前期研究表明芍药甘草汤水提液可降低子宫腺肌病细胞ERαmRNA，故本项目拟通过建立子宫腺肌病痛经患者的病灶细胞体外体系，筛选其增生肥大的子宫平滑肌细胞，通过体外细胞培养实验观察芍药甘草汤水提液对子宫腺肌病痛经患者的病灶细胞增殖活性的干预作用，采用ELISA、免疫细胞化学、RT-PCR等法检测其对细胞ER、BKCa表达的调控，分别在基因水平、蛋白表达等环节探讨芍药甘草汤"一点多位"协同增效产生"多靶点"效应的深层作用机制，旨在阐明中药经方芍药甘草汤止痛的深层作用机理，为子宫腺肌病痛经的新药研发提供实验依据及新的研究思路。

Adenomyosis has gradually become a common gynecological disease, difficult disease, and the incidence of its dysmenorrhea as high as 77.8 %. It seriously affects the quality of life of patients. The adenomyosis which with the dysmenorrhea is with the relevant uterine smooth muscle cells by ER and BKca, TRPV-1 ion channels in the abnormal control of excessive contraction. Our previous studies have shown that SGD can reduce the ERα mRNA of adenomyosis cells. So this project is to screen hypertrophy of uterine smooth muscle cells, in vitro cell culture experimental observation peony and licorice soup extracts the adenomyosis dysmenorrhea patients with lesions of proliferative activity intervention effect, using ELISA, immunocytochemistry, RT-PCR method to detect the expression of ER, of BKCa. Respectively, at the genetic level protein expression and other sectors to explore the Effect of the peony and licorice soup, seeks to clarify the deep mechanism of traditional Chinese medicine by the SGD, to provide experimental evidence and new research ideas.