Potential mechanisms of an antiadenomyosis chinese herbal formula shaoyao-gancao decoction in primary cell culture model.
Potential mechanisms of an antiadenomyosis chinese herbal formula shaoyao-gancao decoction in primary cell culture model.
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抗子宫腺肌病中药方芍药甘草汤在原代细胞培养模型中的潜在机制
DOI：
10.1155/2014/982913
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Su ZR
中科院分区：
文献类型：
--
作者：
Guan YG;Liao JB;Li KY;Li YC;Song Y;Ling J;Su ZR
背景芍药甘草汤是治疗子宫腺肌症、痛经、腹痛、炎症等疾病的著名中药方剂。然而，SGD治疗子宫腺肌病有效性的机制仍然不清楚。本研究旨在利用培养的人子宫腺肌病细胞研究SGD的生物活性及其潜在的分子机制。方法.在体外用SGD及其主要成分芍药苷和甘草苷处理人子宫腺肌病细胞。采用MTT法和流式细胞仪检测甘草苷、芍药苷和SGD对细胞增殖和凋亡的影响。采用ELISA法检测SGD、芍药苷和甘草苷对前列腺素E2（PGE 2）和前列腺素F2 α（PGF 2 α）生成的影响。还通过实时qRT-PCR评价ER-α和OTR mRNA表达水平。结果SGD、芍药苷和甘草苷以剂量依赖性方式抑制人子宫腺肌病衍生细胞的增殖并诱导其凋亡。SGD和芍药苷显著降低PGE 2和PGF 2 α的产生。并能显著降低ER-α和OTR的mRNA水平。结论.本研究结果为SGD治疗子宫腺肌病的生物活性提供了可能的机制，并有助于对该处方的民族药理学知识。
Background. Shaoyao-Gancao Decoction (SGD), a well-known traditional Chinese medicine prescription, has been widely used to treat adenomyosis, dysmenorrhea, abdominal pain, and inflammation in Asia. However, the mechanism underlying the effectiveness of SGD in the treatment of adenomyosis still remains elusive. The present study aimed to investigate the bioactivity of SGD and its underlying molecular mechanisms using cultured human adenomyosis-derived cells. Methods. Human adenomyosis-derived cells were treated with SGD and its major constituents (paeoniflorin and liquiritin) in vitro. Effects of SGD, paeoniflorin, and liquiritin on cell proliferation and apoptosis were examined by MTT assay and flow cytometry analyses. The effects of SGD, paeoniflorin, and liquiritin on the production of PGE2 and PGF2α were assayed using ELISA. ER-α and OTR mRNA expression levels were also evaluated by real-time qRT-PCR. Results. SGD, paeoniflorin, and liquiritin inhibited proliferation and induced apoptosis of human adenomyosis-derived cells in a dose-dependent manner. SGD and paeoniflorin significantly reduced the PGE2 and PGF2α production. Furthermore, they remarkably decreased the mRNA levels of ER-α and OTR. Conclusions. The results of this study provide possible mechanisms for the bioactivity of SGD for treating adenomyosis and contribute to the ethnopharmacological knowledge about this prescription.
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影响因子：
2.2
作者：
Chen, Zheng-ai;Wang, Jun-Long;Bian, Guang-Xing
通讯作者：
Bian, Guang-Xing
影响因子：
6.1
作者：
Kitawaki, J;Obayashi, H;Honjo, H
通讯作者：
Honjo, H
影响因子：
2.7
作者：
Hsu, FL;Lai, CW;Cheng, JT
通讯作者：
Cheng, JT
DOI：
10.1016/j.ejogrb.2007.01.015
发表时间：
2008-04-01
影响因子：
2.6
作者：
Kissler, S.;Zangos, S.;Kaufmann, M.
通讯作者：
Kaufmann, M.
DOI：
10.1016/s1051-0443(99)70213-7
发表时间：
1999-10-01
影响因子：
2.9
作者：
Goodwin, SC;McLucas, B;DeLeon, M
通讯作者：
DeLeon, M