在当前鱼粉资源紧缺的现实困境下，提高鱼类对饲料脂肪的降解和利用效率，降低饲料蛋白质的分解供能比例是节约蛋白质、提高饲料效率的重要途径之一。最新研究提示，鱼体对饲料脂肪的降解和吸收是肠道细胞及肠道微生物互作的结果，但其具体过程和机制尚不明了。为更深入地阐明鱼类对饲料脂肪的消化吸收机制，本项目拟借助学科交叉优势，从肠道微生态角度入手，以罗非鱼和斑马鱼为对象，重点研究肠道微生物及肠道上皮细胞在鱼体降解和吸收饲料脂肪过程中的功能及协同机制。拟首先筛选出罗非鱼肠道中降解油脂的微生物，并将其接种至无菌斑马鱼中，探讨其如何参与宿主对饲料脂肪的降解，以及降解产物对鱼肠道上皮细胞结构、生理功能的影响。其次在活体和离体两个层面，解析目标肠道微生物与肠道上皮细胞在消化、利用饲料脂肪中的协同机制。该项目将为阐明鱼类及鱼肠道微生物对饲料脂肪的消化吸收机制，并为建立鱼类脂代谢人工调控策略提供必要的理论参考。

The shortage of fishmeal and fish oil is the most important global issue for aquaculture. Reducing the energy supply from protein by improving the utilization efficiency of triglyceride in fish is important for promoting the sustainable and healthy development of aquaculture. Both fish and their gut microbiota are involved in the lipid metabolism but the mechanism remains unknown. This study will illustrate the function of intestinal bacteria and epithelial in triglyceride degradation and absorption. First, we will screen oil-degrading bacteria from the intestine of Tilapia and inoculate these bacteria into germ-free zebrafish to identify their function in triglyceride degradation and fatty acids absorption. Then, we will evaluate the synergistic mechanism of the intestinal bacteria and epithelial in triglyceride degradation and absorption in vitro and in vivo in Tilapia. This study will identify the role of fish and fish gut microbiota in the lipid metabolism, providing a theoretical basis for artificial regulation of fish lipid metabolism.