开花是许多高等植物生命周期循环的一个重要过程。作物的地理分布主要取决于光周期调控的开花时间差异。在相同光周期条件下，温度变化极大影响开花时间，因此光周期和温度协同调控开花时间的机制研究具有重要意义。我们前期的研究发现蓝光受体CRY1在温度响应中具有重要功能（PNAS，2016），蓝光和温度都可以调控COR27和COR28的表达而调控开花（Plant Cell 2016），以及升高温度（28ºC）可以互补cry2突变体在长日照条件下的晚花表型，表明温度和蓝光可以协同调控开花时间。本项目拟在前期研究基础上运用基因表达谱分析及拟南芥遗传学突变体筛选分析的方法获得介导蓝光与温度协同调控开花时间的关键新基因，并对所获得关键基因进行系统性功能研究，最终揭示蓝光与温度协同调控光周期诱导开花时间的分子机制。

Flowers are the reproductive organs of plants, flowering is a very important process of plant life cycle. The timing of the transition from a vegetative to reproductive phase is critical for reproductive success in plants. The regulation of flowering time has been a long-term interest for the agricultural research field. The photoperiodic flowering controls the geographical distribution of the crops, while ambient growth temperature also affects flowering, how do light signal and temperature coordinate to regulate flowering is very important for the agriculture. Blue light photoreceptor cryptochrome (CRY) is a key component of photoperiodic flowering, our recent data indicates that CRY1 is critical for the temperature responses (PNAS 2016), and blue light and temperature regulated COR27/COR28 are involved in flowering (Plant Cell 2016), and that higher temperature can complement the late flowering phenotype of cry2 mutant in long day, these results indicate that blue light and temperature coordinate to regulate flowering time. In this project we will try to identify key genes that mediate light and temperature regulated flowering by using Arabidopsis mutant screening and genome wide expression analysis. We then will study the function of those key genes, and uncover the mechanism of how do light and temperature coordinate to regulate photoperiodic flowering.