亚砷酸（As2O3)用于治疗急性早幼粒细胞白血病（APL），其临床应用的不良反应引起人们的普遍关注，其中危及生命的凝血紊乱是其主要的副作用之一。生育三烯酚（Tocotrienols）是维生素E的一个亚族，具有明显的抑制肿瘤作用，对不同种系来源和不同组织来源的肿瘤细胞均有抑制增殖和诱导凋亡作用，且对正常组织细胞无明显毒副作用。本研究拟从离体和整体研究入手，通过细胞实验检测联合给药对APL细胞系NB4细胞凋亡及人脐静脉内皮细胞（HUVEC）凝血活性及微粒的影响，阐明其作用机制；通过建立接种NB4细胞动物模型，观察联合给药对白细胞计数、白血病细胞百分比及其侵润等影响，并检测凋亡相关蛋白表达及凝血情况，验证离体试验结果。明确生育三稀酚和亚砷酸联合给药是否具有增加亚砷酸疗效及降低凝血紊乱作用，明确其作用机制。此项研究将进一步明确并完善生育三烯酚的抗肿瘤作用及其机制，为其合理开发提供理论依据。

Arsenic trioxide (As2O3) has been widely used for the treatment of acute promyelocytic leukemia (APL) . However, the clinical application of adverse reactions caused widespread concern, including life-threatening blood coagulation disorder is one of the main side effect. Tocotrienols is a subgroup of vitamin E, with a significant inhibition of tumor effect on different kinds of sources and tumor cell lines derived from different tissues, and with no significant side effects of normal tissues. In current study, we will examine the apoptosis of NB4 cells , coagulation activity and particulate secretion of human umbilical vein endothelial cells treated with tocotrienols in combination with As2O3. In a NB4 cells xenograft model, we will determine the White blood cell count, percentage of leukemia cells and its invasion. We will also examine the expression of the apoptosis proteins and the coagulation effects. These results will confirm the results from in vitro studies. We will explore whether tocotrienols increases curative effect and decreses the coagulation disorder effect of As2O3 and the futher mechanism. Thus, our study will clear and impove the machanisms of prevention and inhibition of tumor, and apply supporting evidences for development and utilization of tocotrienols in the further.