Mip1b调控上皮器官腔道形态发生的分子机制

目前对腔道器官腔道形态发生的机理认知十分有限。通过对斑马鱼早期胚胎基因表达谱的分析与筛选，我们发现了一个在管腔型器官中高表达的基因，将其命名为Mip1b，并发现这个基因对管腔型器官的形态发生起到重要作用。我们设计获得了该基因的特异Morpholino和蛋白突变体，将其注射到斑马鱼胚胎中，发现当这个基因被下调和蛋白功能被干扰后，神经管中脑脑室腔变小、后脑脑室几乎消失，而且前、后脑的神经管组织中部的弯曲程度变弱，中脑组织中部完全没有弯曲；此外，肾管、小肠以及胰腺的管腔发育异常。综合前期结果，Mip1b调控多种上皮性腔道器官腔道的形态发生。本申请计划研究Mip1b调控腔道形态发生的分子细胞机制。我们的研究结果将为调控腔道器官形态发生提供新的认知和理论

In a gene profile performed on zebrafish during early stage of embryogenesis, we mainly focused on the tubular organisms and related genes whose expression was raised during development. And we have found a new gene that was highly expressed in this process, which was named Mip1b and selected as the interested gene we do research on. Our results showed that the tubular organs developed abnormally in zebrafish embryo injected Mip1b morpholinos (Mip1b morphants) can knockdown Mipb1 function. In the neural tube, the ventricular zone of mid-brain became narrower, and the hind-brain almost disappeared. In addition, pronephric duct, gut and pancreas all showed abnormal development in Mip1b morphants. These results indicate that Mip1b plays an important role in tubular organs during embryogenesis.In this case, we are making a comprehensive plan to address the followingquestions:What is the molecular mechanism of Mip1b regulating the development oftubular organisms.