白桦芽休眠过程中生长素信号转导的分子机理研究

芽休眠是多年生木本植物适应季节变化的主要途径之一，由植物体内多种激素协调控制，其中生长素起关键作用。Aux/IAAs与ARFs以二聚体形式控制生长素信号的转导。本项目将明确在白桦芽休眠过程中，控制生长素信号转导的BpAux/IAA与BpARF基因。从已测序的白桦基因组中鉴定BpAux/IAAs，通过RNA-seq分析这些基因在白桦不同部位中的表达模式，鉴定出在顶芽中高表达的BpAux/IAAs，通过转基因的方法验证所筛选的BpAux/IAAs的功能。通过酵母双杂交筛选与其互做的BpARFs，并通过BiFC进一步验证酵母双杂交的结果。通过转基因技术验证所筛选的BpARFs的功能后，通过ChIP-seq与RNA-seq筛选所获得的BpARFs的下游靶基因。本项目将揭示白桦芽休眠过程中，生长素信号转导的分子机理，具有重要理论意义及应用价值。

Bud dormancy is the main strategy of perennial woody plants to adapt seasonal changes. The bud dormancy of plants is controlled by plant hormones and auxin plays a key role in this biological process. Aux/IAAs and ARFs function as heterodimer in auxin signal transduction pathway. In this project, we will identify Aux/IAAs and ARFs involved in bud dormancy of birch. We will identify BpAux/IAAs with high expressional levels in birch apical bud using RNA-seq, and then validate their functions using transgenic method. Once we identify the BpAux/IAAs involved in bud dormancy of birch, we will scan the interacted BpARFs using Y2H method. We will then validate the results of Y2H using BiFC in birch, and analyze the functions of these BpARFs. Finally, we will identify the target genes of the validated BpARFs. Our results will reveal the molecular mechanism of auxin transduction in bud dormancy of birch. This project have important theory significance and the practical application value.