结直肠癌是一种消化系统恶性肿瘤，在全世界范围内具有较高发病率和死亡率，其中患者死亡的主要原因是由于肿瘤发生了肝转移。探讨和分析结直肠癌肝转移的分子机制，对提高患者的生存率以及早期的诊断与治疗具有重要意义。本课题组前期的研究中通过单细胞测序发现了一种新的GATA家族蛋白TRPS1的突变与结肠癌肝转移密切相关，并且该蛋白与结肠癌远处转移的关系目前尚未有报道。初步研究结果显示TRPS1在多种结肠癌细胞系中高表达，细胞表型实验与动物实验显示TRPS1及其突变体能够显著的促进结肠癌细胞的转移能力。由此推测TRPS1及其突变体在结直肠癌肝转移中会发挥重要的作用。本项目将在以上的研究基础上利用Chip-seq,RNA-Seq等多种分子生物学实验技术以及临床标本、点突变敲入小鼠来系统的阐明该基因在结肠癌肝转移中的分子机制，并且探索该蛋白成为治疗靶点的可行性，为完善结肠癌肝转移的发病机理提供有效证据。

Colorectal cancer is a malignant tumor of the digestive system with a high incidence and mortality worldwide, and the main cause of death in patients because of liver metastasis from tumors. Exploring and analyzing the molecular mechanism of liver metastasis of colorectal cancer will be of great significance for its early diagnosis and improvement of patient survival rate. In the previous study of our group, a new GATA family protein TRPS1 mutation was closely related to colon cancer liver metastasis by single cell sequencing, and the relationship between this protein and distant metastasis of colon cancer has not been reported yet. Preliminary results show that TRPS1 is highly expressed in a variety of colon cancer cell lines. Cell phenotypic experiments and animal experiments show that TRPS1 and its mutants can significantly promote the metastatic ability of colon cancer cells. It is speculated that TRPS1 and its mutants will play an important role in liver metastasis of colon cancer. Based on the above experiments, this project will use the molecular biology experimental techniques such as Chip-seq, RNA-Seq and clinical specimens and point mutation mouse to systematically elucidate the molecular mechanism of this gene in liver metastasis of colon cancer. And explore the feasibility of this protein as a therapeutic target, and provide effective evidence for improving the pathogenesis of colon cancer liver metastasis.