TaIAA21调控小麦籽粒发育的分子路径解析

批准号:
31971930
项目类别:
面上项目
资助金额:
58.0 万元
负责人:
李爱丽
依托单位:
学科分类:
作物基因组及遗传学
结题年份:
2023
批准年份:
2019
项目状态:
已结题
项目参与者:
李爱丽
国基评审专家1V1指导 中标率高出同行96.8%
结合最新热点,提供专业选题建议
深度指导申报书撰写,确保创新可行
指导项目中标800+,快速提高中标率
微信扫码咨询
中文摘要
植物激素生长素在籽粒发育过程中发挥重要作用,然而其作用的分子路径并不十分明确,在小麦中更是空白。在上一期项目资助下,我们利用合成六倍体筛选到一个在籽粒发育早期高表达、突变后导致粒长和粒重增加的AUX/IAA基因TaIAA21。酵母双杂实验表明TaIAA21与花后4天籽粒中特异高表达的TaARF12互作,暗示该互作复合体可能参与小麦籽粒发育调控。为解析生长素在小麦籽粒发育中的分子路径,本项目申请拟从TaIAA21-TaARF12互作出发,采用Co-IP等方法进一步明确其互作关系;结合RNA-seq和ChIP-PCR等方法确定TaARF12的直接调控下游基因,并通过过表达和功能缺失实验阐明TaARF12及其下游基因的功能;在小麦微核心种质中筛选上述重要基因的优异等位变异,并开发可用于育种选择的分子标记。以上工作将阐明TaIAA21基因在籽粒发育中的分子路径,为小麦高产育种提供重要的分子依据。
英文摘要
The phytohormone auxin plays an important role in grain development, but the molecular pathway of its action is not very clear, especially in wheat. Under the support of the previous NSFC project, we found an AUX/IAA gene, TaIAA21, during synthetic wheat study that was highly expressed during early grain development. Mutation of this gene resulted in increased grain length and grain weight. We further found that TaARF12 specifically highly expressed in the grain of 4 days after flowering. Yeast two-hybrid assay showed that TaARF12 interacted with TaIAA21, suggesting that this complex may be involved in wheat grain development. To further dissect the molecular pathway mediated by TaIAA21, we propose in this project to use the observation of TaIAA21-TaARF12 interaction as a starting point firstly to confirm this interaction by using BiFC and Co-IP methods. We will then generate overexpression lines and loss of function lines (by gene editing) TaARF12 so as to reveal the importance of TaIAA21-TaARF12 interaction in grain development. Moreover, RNA-seq, yeast one-hybrid, EMSA and ChIP-PCR will be used to determine the direct regulated downstream genes of this protein complex. Selected downstream genes will be further characterized by genetic analysis (over expression and CRISPR) to clarify their important roles in wheat grain development. Furthermore, elite allelic variation of these important candidate genes will be investigated among accessions of wheat mini-core collection which has recently been re-sequenced and closely linked marker will be developed for breeding. The work highlighted in this proposal will further our understanding of the auxin signaling pathway in wheat grain development and contribute to wheat yield breeding in our country.
期刊论文列表
专著列表
科研奖励列表
会议论文列表
专利列表
DOI:10.1111/tpj.15541
发表时间:2021-11-10
期刊:PLANT JOURNAL
影响因子:7.2
作者:Jia, Meiling;Li, Yanan;Li, Aili
通讯作者:Li, Aili
Transcriptome Analysis of Developing Wheat Grains at Rapid Expanding Phase Reveals Dynamic Gene Expression Patterns.
在快速扩展阶段发育的小麦晶粒的转录组分析揭示了动态基因表达模式。
DOI:10.3390/biology11020281
发表时间:2022-02-10
期刊:Biology
影响因子:4.2
作者:Guan J;Wang Z;Liu S;Kong X;Wang F;Sun G;Geng S;Mao L;Zhou P;Li A
通讯作者:Li A
DOI:10.3389/fpls.2022.850302
发表时间:2022
期刊:FRONTIERS IN PLANT SCIENCE
影响因子:5.6
作者:Wang, Zhenyu;Tao, Shu;Liu, Shaoshuai;Jia, Meiling;Cui, Dada;Sun, Guoliang;Deng, Zhongyin;Wang, Fang;Kong, Xingchen;Fu, Mingxue;Che, Yuqing;Liao, Ruyi;Li, Tao;Geng, Shuaifeng;Mao, Long;Li, Aili
通讯作者:Li, Aili
DOI:10.3390/ijms23105587
发表时间:2022-05-17
期刊:International journal of molecular sciences
影响因子:5.6
作者:
通讯作者:
DOI:--
发表时间:2023
期刊:Plant Biotechnology Journal
影响因子:--
作者:Kong X;Wang F;Wang Z;Gao X;Geng S;Deng Z;Zhang S;Fu M;Cui D;Liu S;Che Y;Liao R;Yin L;Zhou P;Wang K;Ye X;Liu D;Fu X;Mao L;Li A
通讯作者:Li A
小麦重要驯化基因Q调控脱粒性、穗型和穗粒数的遗传基础解析
- 批准号:32172050
- 项目类别:面上项目
- 资助金额:58万元
- 批准年份:2021
- 负责人:李爱丽
- 依托单位:
利用新合成异源六倍体挖掘小麦粒长重要候选基因及其功能研究
- 批准号:31571665
- 项目类别:面上项目
- 资助金额:64.0万元
- 批准年份:2015
- 负责人:李爱丽
- 依托单位:
小麦TaAGO4a参与白粉病抗性的分子路径解析
- 批准号:31271716
- 项目类别:面上项目
- 资助金额:75.0万元
- 批准年份:2012
- 负责人:李爱丽
- 依托单位:
小麦钙依赖性蛋白激酶TaCPK3的功能研究
- 批准号:30771336
- 项目类别:面上项目
- 资助金额:29.0万元
- 批准年份:2007
- 负责人:李爱丽
- 依托单位:
国内基金
海外基金
