地下害虫蛴螬是导致全球作物、牧场和森林遭受严重经济损失的重要因素，而其与植物和根际细菌三者互作研究尚属空白。申请人前期研究发现，蛴螬为害初期，花生根际细菌群落结构发生显著变化，富集了对鞘翅目具有杀虫活性的肠杆菌属（Enterobacter spp.）等菌株，而这些丰度增加的菌株对鳞翅目害虫小菜蛾却无杀虫活性。本项目拟利用已建立的“蛴螬-花生-根际微生物”互作平台，通过蛴螬为害初期花生根部代谢组及转录组数据，寻找差异代谢物，并分析其对富集杀虫菌株趋化性等影响；同时在花生根系中沉默关键代谢物合成途径基因，明确关键代谢物功能，解析虫害诱导植物富集具有杀虫活性菌株的机制。预期结果对突破现有杀虫菌株筛选模式，建立新型高效、环境适应性强的生防菌株筛选体系提供理论指导。对于充分挖掘作物根际微生物资源、减少化学农药的使用具有重要意义。

Subterranean pests, white grubs, are an important factor leading to severe economic losses in crops, pastures and forests around the world, and their interaction with plants and rhizosphere bacteria is still unknown. In previous investigation, the applicant found that the bacterial community structure of peanut rhizosphere changed significantly in the early stage of white grub hazard, and enriched strains such as Enterobacter spp., which have insecticidal activity against Coleopteran insect pests, and these strains with increased abundance showed no insecticidal activity against Lepidoptera insect pests, such as Plutella xylostella. The project intends to use the established “white grub-peanut-rhizosphere microbial” interaction platform to find differential metabolites through the metabolome and transcriptome data of the peanut roots in the early period of pest damage, and analyze the effects on the chemotaxis of enriched insecticidal strains; silence key genes of metabolite synthesis pathway in peanut roots, verify key metabolite functions to clarify the mechanism by which insects induce plant enrichment of insecticidal strains. The expected results will provide theoretical guidance for breaking through the existing screening mode of insecticidal strains and establishing a new high-efficiency, environmentally adaptable biocontrol strain screening system. It is of great significance for fully exploiting the rhizosphere microbial resources of crops and reducing the use of chemical pesticides.