申请人前期研究发现Tween 40不仅能下调钝齿棒杆菌dtsR1转录、降低ODHC酶活，并能显著上调zwf转录、促进NADPH供应并上调精氨酸合成途径中argB的转录，提高精氨酸发酵水平；同时发现菌株在敲除dtsR1后，ODHC酶活进一步下降，Tween 40仍能上调zwf和argB基因的转录，提高精氨酸产量；但Tween 40与dtsR1是如何影响钝齿棒杆菌精氨酸生物合成以及二者之间的关联性如何，仍有系列问题亟待探索。针对以上新问题，申请人拟采用转录组和蛋白质组这两方面互补和整合式手段系统探讨Tween 40是如何影响dtsR1及精氨酸合成网络相关基因的转录？如何影响NADPH供应？以及DtsR1如何调控pknG、ppp转录以及OdhI磷酸化等。总之，本课题旨在全面系统地解析Tween 40及DtsR1影响钝齿棒杆菌产精氨酸的作用机制。

Our previous work have demonstrated that Tween 40 could not only downregulate the dtsR1 transcription; lower ODHC activity; upregulate the zwf transcription and promote the NADPH supply, but also increase the transcriptional level of argB, which is encoded the key enzyme of arginine biosynthetic pathway for improvement of arginine production in Corynebacterium crenatum. After knocked out the dtsR1, the ODHC activity was found to further decline in Corynebacterium crenatum, whereas the transcriptional level of zwf and argB were upregulated by the addition of Tween 40. However, there are still a series of problems to be explored that how the Tween 40 and DtsR1 effect the arginine biosynthesis in Corynebacterium crenatum. In the present study, the applicant intends to use the transcriptome and proteome techniques to explore the mechanism of Tween 40 and dtsR1 on the related gene transcriptions of arginine biosynthetic network; Tween 40 on the NADPH.supply; and how the DtsR1 regulate pknG and ppp transcriptional levels and OdhI phosphorylation, and so on. In a word, this proposal is aimed to comprehensively explain the mechanism of Tween 40 and DtsR1 on L-Arg biosynthetic network in Corynebacterium crenatum.