HIV-1中V3环基因变异与HIV-1的致病性、免疫逃避、细胞嗜性等密切相关，但HAART后对V3的影响仍然存在争议。HAART虽能有效抑制病毒但潜伏感染持续存在（主要宿主细胞为静息型CD4+T淋巴细胞）。目前无“云南HIV/AIDS患者HAART后V3环基因变异性及静息型CD4+T淋巴细胞数量的动态变化”的相关研究。前期已对HIV-1前病毒DNA、T淋巴细胞、HIV-1病毒env准种进行了初步研究，本研究以云南主要流行的HIV-1亚型和重组型为切入点，为临床提供一个较完整的HAART后HIV-1病毒V3环基因变异性及静息型CD4+T细胞数量的动态变化信息。在病毒基因、病毒潜伏感染细胞数量、临床指标的相互联系中，探讨HIV/AIDS临床治疗的基因瓶颈，为优化临床HAART方案和寻找降低HIV-1病毒存储库的治疗策略提供依据。

The sequence variation of HIV-1 V3 loop relate to the pathogenicity,immune evasion,and cell tropism of HIV-1.At the same time, the impact of HAART on V3 is still controversial.Although HAART prevent HIV-1 RNA effectively,a pool of latently infected cells in resting CD4+T cells.There are little research on V3 loop gene variability of HIV-1 and account of resting CD4+T lymphocyte after HAART in Yunnan HIV/AIDS individuals.We have studied on HIV-1 DNA,T lymphocyte,and HIV-1 env quasispecies.This study will study on main HIV-1 subtypes and recombination types in Yunnan,analyze on variability and polymorphism of the HIV-1 V3 loop by bioinformatics,and study on the account changes of resting CD4+T lymphocytes.By studying on correlation of viral genes, the number of latently infected cells and clinical indicators, HIV-1 V3 loop may be proved to be the genetic bottleneck of AIDS treatment.These date will be the information for optimizing HAART and to find treatment strategies for supression of latenet HIV-1 reservoir.