延迟性肌肉酸痛MR功能成像与NGF、mTOR信号传导通路变化特征研究

延迟性肌肉酸痛（DOMS）疼痛机制不清，我们前期课题发现DOMS SD大鼠、志愿者31P-MRS ADP、Pi/ATP、pH（能量、代谢）动态变化与DOMS疼痛时间变化趋势相同，MR-DTI FA、本征值能够反映DOMS可逆损伤组织学特征，但目前疼痛机制学说难以在细胞、分子水平解释DOMS疼痛机制，本课题拟在前期工作基础上研究DOMS SD大鼠、人体模型MR功能影像（31P-MRS，MR-DTI）与其疼痛指标的相关性，分析不同观察点股四头肌、脊髓、背根神经节各自相应的NGF、TrkA、ASIC3、mTOR、p70S6K及缓激肽、IL-6 mRNA和蛋白的表达及其在注入相应拮抗剂或抑制剂后的各种变化，研究MR功能成像、DOMS疼痛相关分子通路的变化及NGF、mTOR在DOMS炎性疼痛学说中的可能参与及调控作用，多角度探讨DOMS 疼痛NGF、mTOR信号通路分子机制，为运动性疼痛诊治奠定基础

The mechanism of DOMS is still unclear. Our previous works showed that the nature course of pain in DOMS is similar to the dynamic changes of ADP, Pi/ATP, pH value in vivo rats and volunteers 31P-MRS, and showed that the FA, eigenvalue in MR-DTI could reflect the histological features of the reversible muscle injury in DOMS. But the pain of DOMS could not be explained by the DOMS pain mechanism theory at the cellular and molecular level nowadays. Based on our previous studies, this research is to find the correlation between MR Functional Imaging (31P-MRS and MR-DTI) in DOMS SD rats and humans with its related pain changings, to analysis the mRNA and protein expression of NGF, TrkA, ASIC3, mTOR, p70S6K, Bradykinin, IL-6 at different time points in quadriceps femoris,spinal cord, dorsal root ganglion respectively, and their changes after injection of its corresponding antagon and inhibitor, further to study MR Functional Imaging, the pain-related molecular transduction in DOMS, and the possible regulatory role of NGF and mTOR in DOMS inflammatory pain mechanism. Our purpose is to explore molecular mechanism of NGF and mTOR signal transduction in pain of DOMS and lay the foundation for clinical diagnosis and management of exercise-induced pain.