能量代谢在维持生物体稳态、调控寿命、以及组织再生中发挥重要功能。线粒体能量代谢在调控生物体寿命以及调控干细胞命运决定中发挥至关重要的作用，但是线粒体在组织再生中的功能还有待进一步阐明。涡虫具有强大的再生能力，近年来已经发展为研究组织再生机制的重要模式动物之一，而涡虫线粒体对干细胞功能的调控的细胞学基础尚不清楚。申请人前期研究提示，通过线粒体染色可以分离出具有多能性的涡虫成体干细胞。高水平线粒体信号的涡虫干细胞失去了再殖功能。基于这些前期研究，申请人将进一步探索线粒体功能对涡虫干细胞命运的影响。利用单细胞测序对比不同线粒体信号的涡虫细胞，精确绘制涡虫成体干细胞的分化谱系，通过线粒体代谢调控基因的差异表达谱系，探索线粒体调控涡虫干细胞命运和组织再生的分子机制。

Energy metabolism plays critical roles in maintaining biological homeostasis, ageing regulation and tissue regenerative process etc. Mitochondrion is an important regulator in controlling ageing and stem cell fate decision. Until recently, how mitochondria regulate tissue regeneration still requires deep understanding. Planarian is an ideal model for investigating tissue regeneration due to its powerful regenerative ability. However, little is known whether mitochondria regulate planarian regeneration as well as the underlying mechanism. Our pilot study indicates mitochondria staining could separate the adult stem cell of planarian. Meanwhile, stem cells with higher mitochondrial signal lost the ability of repopulation. As such, this project will further explore the how mitochondria regulate planarian stem cell fate decision. Single cell sequencing will be employed to clarify the lineage directions of planarian stem cells. This project will compare the mitochondrial regulators among different lineages, and thereby investigate the molecular mechanism underlying the effects of mitochondria to planarian stem cell lineage decision and tissue regeneration.