油菜籽粒种皮颜色是重要的品质性状。黄籽具有种皮薄、油分和蛋白质含量高、色素和纤维素含量低等优点。油菜的种皮颜色受多基因控制，黑籽一般为显性，多个位点都为隐性纯合时才表现为黄籽，所以选育高产优质的黄籽甘蓝型油菜品种非常困难。人工合成的甘蓝型油菜品系No2127-17的籽粒纯黄，其黄籽性状受一个不完全显性基因控制，这种遗传模式明显不同于油菜中的其它黄籽材料。前期的研究中，我们完成了显性黄籽基因的精细定位，构建了No2127-17的BAC文库，并筛选到覆盖黄籽基因区段的BAC克隆。本研究中，我们拟对BAC克隆进行测序，通过与埃塞俄比亚芥、甘蓝和甘蓝型油菜基因组序列相比较，鉴定黄籽候选基因，进而通过转基因、基因表达分析、转录组分析、种皮色素染色和不同发育时期种皮色素成分分析，确定基因的功能及组织表达特异性，阐明该基因控制的代谢途径及其调控规律，揭示显性黄籽性状形成的分子机理，为黄籽育种提供服务。

Seed-coat color is one of the most important quality traits in Brassica napus. Yellow seeds have several advantages over blasck seeds with the same genetic background such as thinner seed-coat, higher oil content, higher protein and lower fiber content. Seed-coat color in B. napus is controlled by multiple genes and its genetic basis is very complex. Black seed-coat is generally dominant over yellow seed-coat. Only when all these loci are recessive homozygous, the seed-coat will be in yellow color. Thus, development of high yielding, high quality and yellow-seeded rapeseed is difficult. No2127-17 is an artificially syntheszed pure yellow-seeded inbred line. Its yellow-seeded trait is controlled by a single semi-dominant gene, which is obviously different from the genetic models of all yellow seed-coat color in B. napus.In previous studies, we fine mapped the yellow seed-coat gene and constructed a BAC library of No2127-17, and identified two BAC clones covering the genomic region of the yellow seed-coat gene.In this study, we are planning to sequence the BAC clones and identify the gene by comparing the genomic sequences from the BAC, B. carinata, B. oleracea and B. napus, and to characterize its function through genetic transformation, gene expression analysis, RNA sequencing, seed-coat pigment staining and metobolites analysis. The goals are to reveal its roles in the biosynthesis of seed-coat pigment and its regulation, and finally reveal the molecular mechanisms of the dominant yellow-seeded trait. We expect that the results of this study could faciliate yellow-seeded rapeseed breeding.