基于液相色谱-质谱联用技术和气相色谱-质谱联用技术的不同表型哮喘患者的血清代谢组学研究

代谢组学通过考察生物体系(细胞、组织或生物体)受刺激或扰动后(疾病或环境变化)其代谢产物的变化来研究生物体系，更能直接反映机体系统的生理和病理状态。哮喘发病机制复杂，涉及到多种不同的细胞类型和大量的通路，产生了多种的代谢产物。本课题组前期应用气相色谱-质谱联用的方法，比较了哮喘患者与健康对照者的血清代谢物谱的差异，结果发现2组血清小分子代谢物的种类和水平存在显著差异，与其他研究的结果一致。但哮喘组患者之间血清代谢谱的个体差异较大，我们推测可能与哮喘存在较多的表型有关。不同表型的病生理机制不同，可能导致不同表型哮喘患者的血清代谢谱存在差异。本研究将应用气相色谱-质谱联用方法、液相色谱-质谱联用方法，分析不同表型哮喘患者之间血清代谢谱的差异并筛选差异代谢物、寻找异常代谢环节。该项目的完成，将有助于从代谢角度探明各表型的发病机制、寻找各表型潜在的代谢标志物并发现各表型潜在的新的治疗靶点。

The aim of metabolomics is to study the biological system(including cells, tissue, or whole organisms) through analyzing the change of metabolites after the biological system has benn perturbed by environmental factors or disease processes. It can reflect the pathophysiologic state more directly. The pathogenesis of asthma is very complicated. Because the pathophysiology of asthma involves many different cell types and many pathways, many metabolites may be produced. In our former study, we have analyzed the serum levels of metabolites in both asthma patients and healthy control by gaschromatographic-mass spectrometry method. Consistant with other studies, we found that the metabolomic frofile of asthma patients was significantly different from healthy control. But the difference within the group is also large. The heterogeneity of asthma may explain the difference within the asthma group. The aim of this study is comparing the metabolomic profile between different phenotypes of asthma, screening for potential biomarkers of each pehnotype and finding abnormal metabolic pathway. This study will promote to clarify the pathophysiology of different phenotype and find new therapy target for each phenotype.