近年来随着对我国蘑菇中毒事件的广泛调查和深入，导致神经中毒的事件越来越多，主要由含毒蕈碱和异噁唑衍生物的丝盖伞属和鹅膏菌属鹅膏组的一些种类所致。在我国，对于这类蘑菇的资源、分类和系统发育研究取得了一定成绩，但是对于这类蘑菇的毒素检测和研究则完全处于空白状态。在前期研究的基础上，本项目拟开展4个方面的研究：（1）通过中毒调查、野外采集收集丝盖伞属和鹅膏菌属鹅膏组标本，进行分子系统发育研究；（2）利用HPLC技术建立毒蕈碱和异噁唑衍生物定性定量方法，检测各物种的毒素成分及含量；（3）利用HPLC-MS鉴定各物种的毒素及相关代谢成分，并依据HPLC指纹图谱进行聚类分析，构建系统树；（4）探讨基于毒素和相关代谢成分的系统树和基于DNA序列的系统树之间的关系。该项目的实施将极大丰富我国毒蘑菇种类及毒素成分，对于了解毒素多样性、化学分类具有重要科学意义，对于我国蘑菇中毒预防和诊断治疗具有重要指导价值。

In recent years, with the extensive investigation of mushroom poisoning in China, more and more cases of nerve mushroom poisoning have been occurred, which were caused mainly by the species of Inocybe and Amanita genus section Amanita containing muscarine and isoxazole derivatives. The research on resources, morphological classification and molecular phylogeny of this kinds of mushrooms have made good achievements. However, the detection and study of toxins in this kinds of mushrooms is completely blank in our country. Based on the previous research progress, this project intends to carry out four aspects of research: (1) to collect the samples of Inocybe and Amanita genus section Amanita and to establish phylogenetic tree based on multi-locus DNA sequence; (2) to establish the qualitative and quantitative methods of muscarine and isooxazole derivatives by means of HPLC methods, and to analyze the toxin components and their contents of mushrooms in China; (3) to identify the toxins and relative metabolites and to establish the fingerprints of toxins and relative metabolites of mushrooms based on HPLC-MS and hierarchical cluster analysis was carried out. (4) to study the relationship between the phylogenetic tree based on toxin component and the phylogenetic tree based on multi-locus DNA sequence. The implementation of this project will greatly enrich the species and components of poisonous mushroom in China. It is of great scientific significance to understand the toxin diversity and chemical classification of the two genera, and has important application guiding value for the prevention of mushroom poisoning, diagnosis and treatment of mushroom poisoning in China.