油桐尺蛾核型多角体病毒宿主域决定的分子机制

油桐尺蛾是我国南方重要的林业害虫，每年都会造成严重的经济损失。油桐尺蛾核型多角体病毒作为其自然天敌，具有高专一性，对人畜、植物、水土及其他天敌昆虫无害的特点，是很好的生物防治制剂。但也是由于其高专一性，其对林间同时期发生的其它害虫无效，并且人工扩繁时只能用油桐尺蛾而没有其它可替代寄主，因此期望通过开发其特定的宿主域来解决这一问题。故本研究以油桐尺蛾核型多角体病毒为研究对象，通过人工重组的方法选育同时对油桐尺蛾和家蚕都具有相对较高毒力的重组病毒，利用高通量测序技术对宿主域扩大的重组病毒及其亲本进行基因组测序，在对比分析基因组数据的基础上明确油桐尺蛾核型多角体病毒重组后的差异基因，进而对其进行功能验证，最终找到其宿主域决定的关键基因，从而阐明其宿主域决定的分子机制。本研究的实施不但能很好地为本领域的后续研究提供充足的基础数据，并且将为进一步用基因工程方法研制高侵染能力的病毒株提供科学依据。

Buzura suppressaria (Bs) is a serious forest pest in south of China, and causes grave economic losses every year. Buzura Suppressaria nucleopolyhedrovirus (BsNPV), which is Bs’ natural enemy, can infect Bs with high specificity, and nearly has no impact on other organism, water and soil. So it is an attractive biocontrol method to promote. But also because of its specificity, it can’t control other pests occur in the same period, and doesn’t have alternative host when we proliferate it in lab artificially, so it is expected that it can expand its host range specifically to solve these issues. This program will take BsNPV as research object. Recombinant virus which has high virulence to both Bs and Bombyx mori (Bm) will be bred artificially. Then the genome of recombinant virus, BsNPV and BmNPV will be sequenced using high-throughput sequencing technology. To find differences between recombinant virus and its parents, genomes will be compared after data assemble. Host range control related genes will be identified based on functional verification. So far the molecular mechanism of BsNPV host range control will be clarified. Implementation of this program can not only supply a large number of basic data to further researches in baculovirus researches, but also provide scientific basis to develop new virus with higher infectility by genetic engineering technology.