干燥储存好氧颗粒污泥致力于快速启动反应器、维持系统长期稳定运行，已成为污水处理领域的研究热点。然而干储颗粒复活中低效的群感响应（QS）严重影响其效能，以往单一信号QS调控手段无法对颗粒复杂的混菌状态进行有效强化，阻碍了该技术的推广。本项目拟以好氧颗粒污泥为研究对象，通过对比分析四种群感信号的特性和传导情况，掌握信号间的协作和串扰规律；通过考察各信号与功能性菌群的有效响应关系，筛选出无串扰的高效信号组合；通过研究孤儿受体菌在信号菌中的分布情况，对其筛选、分离与鉴定，掌握其对异源信号的感应机制；通过添加高效信号、孤儿受体菌群等手段复活干储颗粒，分析颗粒综合性能及活性菌群的变化情况，结合非靶代谢组学与细胞交流基因测序，揭示干储复活中关键代谢步骤及其响应通路，构筑高效的干储颗粒复活法，明确信号间串扰和孤儿受体菌对干储颗粒复活中群感效应的强化调控机制，将为颗粒储存在污水处理领域的推广奠定坚实基础。

Dehydrated storage of aerobic granular sludge is a fast way to start the reactor and maintain the long-term stable operation, while it’s becoming a great potential hot-spot in the field of sewage treatment research. However, the inefficient quorum sensing (QS) of granules in recovery of dehydrated storage affected its effectiveness seriously, which is the main technical obstacle for application. While single quorum sensing signal could not regulate the granules with mixed bacteria state effectively. So according to causes and impact of granules instability after dehydrated storage, optimizing recovery technologies will be presented in this project. The most critical thing is clearing emergency response and regulation between quorum sensing signals and resistant microbes in air-drying storage by comparison with recovery effect of granules in different adversities. While to screen out the high-efficiency signals, interactions and cross-talk between four signals will be studied by cross-study validation and combined analysis. Also, to construct the association and conduction pathways between signals and microbes, the spatial distribution of signal-producing, signal-quenching microbes and resistant microbes will be studied in granulation process. To clarify the induction mechanism of heterologous signals, the distribution of orphan receptor bacteria will be analyzed in the signal consortia, by the way, the orphan receptor microbial consortium will be screened and isolated. To optimize recovery conditions, several strategies including adding the screened high-efficiency signals and orphan receptor microbial consortia will be studied in recovery stage with analyzing the comprehensive performance of the granules and the changes of active flora. To reveal the key metabolic steps and response pathways in recovery of dehydrated storage, it will be analyzed by combining non-target metabolomics and bacterial communication genes sequencing. Finally, to achieve stable regulation of recovery effect, effective recovery conditions of dehydrated storage will be created by optimizing key metabolic steps in the regulation pathway. The proposed new recovery method of granular dehydrated storage and the regulation strategy of emergency response between quorum sensing signals and resistant microbes, can provide the necessary theoretical basis and technical support for granular sludge technology engineering application in the field of sewage biological treatment.