树鼩EB病毒感染模型的创建与评价

EB病毒（EBV）的成人感染率达95%，不仅可导致急性单核细胞增多症，还与多种淋巴瘤及上皮细胞起源的恶性肿瘤的发生发展关系密切。目前尚无可用于EBV特异性治疗和预防的化学疗法或疫苗。缺乏合适的动物模型是制约EBV感染、致瘤机理及疫苗开发等研究的瓶颈。树鼩在进化上与灵长类相近且经济适用，在多种病毒的感染研究中已有应用。课题组前期研究发现树鼩对EBV易感；本课题将进一步完善体内、体外实验，研究感染后病毒生命周期变化、病毒的组织嗜性、消长规律以及树鼩机体免疫应答等特点，以评价树鼩模型的相似性；并在此基础上，对感染EBV的适宜年龄、剂量等条件进行探索，以优化模型的稳定性；最后通过探索建立EBV感染后急性单核细胞增多症模型和感染后应用免疫抑制剂是否发展为淋巴组织增生症模型，以验证树鼩模型的可靠性。本项目的完成将为EBV研究提供新的且稳定、重复性好的动物模型，为EBV的防治奠定基础。

Epstein-Barr virus (EBV), which can not only cause acute mononucleosis, but also closely related to the occurrence and development of multiple lymphomas and epithelial-derived malignancies，has infected 95% of adults in the worldwide. The lack of suitable animal models is a bottleneck restricting research on EBV infection, tumorigenic mechanisms, and vaccine development. Tree shrews are evolutionarily close to primates and economically viable, so they have been used in the study of infection of many kinds of viruses. Previous work from our research group found that tree shrews are susceptible to EBV. This project will further improve the in vivo and in vitro experiments, and study the characteristics of virus life cycle after infection, viral tissue tropism, growth and decline, and the immune response of tree shrews to evaluate the similarity of tree shrew model. On this basis, we will explore the appropriate age and dose of EBV infection to optimize the stability of the model; Finally, we will verify the reliability of tree shrew model by exploring the establishment of acute mononucleosis model after EBV infection and whether the application of immunosuppressant after infection develops into lymphoid tissue hyperplasia model. The completion of this project will provide a new, stable and repeatable tree shrew model for EBV research, and lay a foundation for the prevention and treatment of EBV.